芦荟提取物C诱生小鼠细胞毒因子的研究  

Study on Aloe Extract C induced cytotoxic factors in mice

作　　者：谢光麟[1] 赫甡[1] 赫明昌[1] 

机构地区：[1]中国医科大学基础医学院,辽宁沈阳110001

出　　处：《广东药学院学报》2002年第4期297-299,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：辽宁省科委课题(98225003)

摘　　要：目的:研究芦荟提取物C(Aloe Extract C,AE-C)在BALB/C鼠体内诱生细胞毒因子的作用。方法:连续5 d给小鼠注射AE-C,第6 d取血分离血清,应用结晶紫染色法测其细胞毒因子活性;观察经AE-C诱生的鼠血清对多种瘤细胞的杀伤作用;杀伤作用的持续时间和对温度的敏感性及血清不同稀释度的杀伤效果。结果:AE-C能诱导小鼠产生杀伤活性较强的细胞毒因子,与对照组比较有明显的差异(P<0.01);并且对多种靶细胞有杀伤作用;谤生后24 h达高峰,第4 d下降明显,5 d接近正常水平;血清标本稀释至200倍时,仍具有较强的杀伤活性;诱生后的细胞毒因子有一定的温度敏感性.结论:A-C有诱生BALB/C鼠产生细胞毒因子的作用。Objective: To study the effect of Aloe Extract C ( AE - C) in inducing cytotoxic factors in mice. Methods: Mice were injected with AE - C through abdominal cavity for 5 days, blood was taken for separating serum on the sixth day. Y99 , L929 and A549 tumor cell lines were used as target cells to assay cytotoxic factors activity of the serum. The lasting time of the activity, its sensitivity to temperature and the cytotoxic activity of different concentration of the serum were observed. Results: AE - C could induce the production of cytotoxic factors in BALB/C mice, compared with the control, there was a significant differences ( P < 0. 01 ) . Cytotoxic activity reached the peak 24 hours after last injection of AE - C and began to drop 4 days later. The cytotoxic activity disappeared 5 days later. The cytotoxic acitivity was still obvious with 200 times dilution of the serum. Conclusions: AE - C can induce the production of cytotoxic factors in BALB/C mice.

关 键 词：芦荟 AE-C 细胞毒因子 血清 靶细胞 中药 

分 类 号：R285.5[医药卫生—中药学]