两种不同初筛酶联免疫吸附试验试剂联合检测以替代艾滋病病毒抗体蛋白印迹试验确认检测的对比分析  

Study on Combined Test with Two Kinds of Different Format HIV-Ab ELISAs as a Substitute for Western Blot

作　　者：马义才[1] 秦光明[2] 郑国英[2] 刘莉[2] 张灵麟[2] 刘刚[2] 毛晓英[2] 

机构地区：[1]中国医学科学院中国协和医科大学输血研究所,四川成都610081 [2]四川省卫生防疫站

出　　处：《中国性病艾滋病防治》2002年第6期335-337,356,共4页Chinese Journal of Std & Aids Prevention and Control

摘　　要：目的　为评价两种不同初筛酶联免疫吸附试验 (ELISA)试剂联合检测艾滋病病毒 (HIV)抗体的可靠性 ,以替代艾滋病病毒 (HIV)蛋白印迹 (Westernblotting ,WB)确认法。 方法　79份初检为HIV抗体阳性的吸毒人员血清 ,重新统一用两种抗体初筛ELISA试剂 (Vironostika○RHIVUni FormⅡ plus 0和UBIHIV 1 / 2EIA)进行复测 ,之后随机选择前 51份样品进一步做WB确认 ,并做对比分析。 结果　51份血清中 ,有 49份样品 2次初筛复测时至少有 1次复测的结果显示为S/CO≥ 6 ,它们的WB确认结果均为HIV抗体阳性 ;其余的 2份样品 2次初筛复测时 ,其S/CO均 <6 ,但其中 1份 (样品“0 5”)的WB结果为HIV抗体阴性 ,1份 (样品“584”)为HIV抗体阳性。确认为HIV抗体阴性的样品“0 5” ,在整个初筛的 3次检测中曾 2次出现过S/CO >1 ;确认为HIV抗体阳性的样品“584” ,虽然 3次初筛检测中S/CO值均 >1 ,但因本试验中 2次复测得到的S/CO都 <6 ,因而其确认结果亦显示出较少抗原带 ,即只有p2 4和Gp1 60。 结论　两种不同原理的ELISA试剂 (最好为进口试剂 )联合检测HIV抗体可替代WB确认法 ,以监测具有一定HIV流行率的风险人群 (如哨点监测人群 )的HIV/艾滋病疫情。报告为HIV抗体阳性的临界判断指标采用 2次初筛S/CO≥ 6应是可靠的 ;Objective To evaluate the reliability of combined test with two kinds of different format ELISAs as a substitute for Western blot Methods Seventynine drug users' ELISA positive sera were re-tested with two kinds of HIV-Ab ELISAs(Vironostika D○R Uni-Form Ⅱ Plus O and UBI HIV-1/2 EIA)and then the first 51 ELISA positive sera were confirmed with Western blot Results Western blot demonstrated that 49 sera with S/CO≥6 in ELISA re-testings were postitive to HIV antibody and 2 sera('05'and'584')with S/CO<6 in ELISA re-testings were HIV-Ab negative and positive respectively Serum'05'had both of S/CO<1 among three ELISA testings Serum'584'had fewer confirmatory test-trips(only p24 and gp160)because of its S/CO<6 in two re-testings Conclusions The combined testing method with two kinds of different format HIV-Ab ELISAs,especially the imported third-generation reagent,may be used as a replacement of Western blot for epidemic surveillance of some HIV risk populations S/CO≥6 of the combined ELISAs,as a critical quota of HIV-Ab positive results,may be reliable; the sera with 6>S/CO≥1 in the combined ELISA testing need to be further confirmed by Western blot; when a result needs to be informed to the patient himself,it will be necessary to carry out Western blot assay, though the combined ELISA test is shown to be positive to HIV antibody

关 键 词：酶联免疫吸附试验 蛋白印迹试验 S/CO 替代 HIV 

分 类 号：R512.91[医药卫生—内科学]