依文思蓝褪色分光光度法测定雷洛昔芬  

Fading Spectrophotometric Method for the Determination of Raloxifene with Evans Blue

作　　者：秦宗会[1] 范莉[1] 刘绍璞[1] 江虹[1] 

机构地区：[1]西南师范大学环境化学研究所

出　　处：《分析化学》2002年第12期1486-1489,共4页Chinese Journal of Analytical Chemistry

摘　　要：在pH 1 .4～ 2 .5的NaAc HCl缓冲介质中 ,雷洛昔芬 (raloxifene)与依文思蓝 (evansblue)反应形成离子缔合物 ,使依文思蓝溶液褪色 ,最大褪色波长位于 61 0nm。雷洛昔芬在 0～ 1 .4× 1 0 - 5mol/L的浓度范围内遵守比尔定律 ,表观摩尔吸光系数ε6 1 0 为 1 .76× 1 0 4L·mol- 1 ·cm- 1 。In sodium acetate-hydrochloric acid buffer solution at pH 1.4 similar to 2.5, raloxifene (Ralo) reacts with evans blue (EB) to form an ion-association complex, which make the EB fade. The measurement wavelength for the spectrophotometric deermination of Ralo is at 610 nm. Beer's law is obeyed in the range of 0 similar to 1.4 x 10(-5) mol/L, and the apparent molar absorptivity epsilon(610) is 1.76 x 10(4) L.mol(-1).cm(-1). The method has high sensitivity and fairly good selectivity.

关 键 词：褪色分光光度法 测定 雷洛昔芬 依文思蓝 雌激素药物 药物分析 

分 类 号：O657.3[理学—分析化学]