通过三嗪荧光探针的多氢键作用识别胸腺嘧啶  

Recognition of Thymine by Triazinyl Fluorescence Probe Through the Formation of Intermolecular Multi-hydrogen Bonds

作　　者：聂丽华[1] 马会民[1] 李晓花[1] 孙铭[1] 尤洪涛[1,2] 金永平[1,3] 

机构地区：[1]中国科学院化学研究所分子科学中心,化学生物学联合实验室,北京100080 [2]北京市烟草专卖局 [3]浙江省环境保护科学设计研究院

出　　处：《高等学校化学学报》2003年第1期37-39,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 175 0 31;2 0 0 35 0 10 )资助

摘　　要：In this work, a novel long-wavelength fluorescence probe, 3-(4-chloro-6-methylamino-1,3,5-triazinylamino)-7-dimethylamino-2-methylphenazine, was synthesized by the reaction of neutral red and methylamine subsequently with cyanuric chloride, and its recognition behavior for thymine together with its spectroscopic properties in different solvents was studied. The results show that the fluorescence probe can be selectively quenched by thymine instead of guanine, suggesting that the fully complementary hydrogen bonding plays a key role in such a recognition process.In this work, a novel long-wavelength fluorescence probe, 3-(4-chloro-6-methylamino-1,3,5-triazinylamino)-7-dimethylamino-2-methylphenazine, was synthesized by the reaction of neutral red and methylamine subsequently with cyanuric chloride, and its recognition behavior for thymine together with its spectroscopic properties in different solvents was studied. The results show that the fluorescence probe can be selectively quenched by thymine instead of guanine, suggesting that the fully complementary hydrogen bonding plays a key role in such a recognition process.

关 键 词：三嗪 分子间氢键 荧光探针 分子识别 胸腺嘧啶 核酸 碱基 

分 类 号：Q526[生物学—生物化学]