辅酶NADH模型物还原反应动力学的核磁共振研究  

A Kinetic NMR Study of Reduction Mechanism of NADH Models

作　　者：李劲[1] 刘有成[1] 吴赛玉[2] 夏静静[1] 张家海[2] 吴季辉[2] 

机构地区：[1]中国科学技术大学化学系,合肥230026 [2]中国科学技术大学生物系,合肥230026

出　　处：《高等学校化学学报》2003年第1期73-75,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 0 72 0 36 ;2 9832 0 40 );中国科学技术大学青年基金资助

摘　　要：The rate constant and kinetic isotopic effect(k H/k D) of the reduction of 2-bromo-1-phenylethylidenemalononitrile with NADH model were determined through NMR spectroscopy. The results were consistent with that the hydride transfer step of the reaction which was the rate-determining step, and were comparable to the results obtained through UV-Vis Spectroscopy reported previously.The rate constant and kinetic isotopic effect(k H/k D) of the reduction of 2-bromo-1-phenylethylidenemalononitrile with NADH model were determined through NMR spectroscopy. The results were consistent with that the hydride transfer step of the reaction which was the rate-determining step, and were comparable to the results obtained through UV-Vis Spectroscopy reported previously.

关 键 词：辅酶NADH模型物 还原反应 动力学 核磁共振 负氢转移 

分 类 号：Q552[生物学—生物化学]