肾上腺素预处理对大鼠离体心脏缺血再灌注的保护作用  

Preconditioning of epinephrine for myocardial protection after ischemia-reperfusion injury in isolated rat heart

作　　者：施东伟[1] 高峰[1] 姚晨玲[1] 童朝阳[1] 黄培志[1] 陈百华[1] 洪慧慧[1] 

机构地区：[1]复旦大学附属中山医院急诊科,上海200032

出　　处：《上海医学》2002年第12期755-757,共3页Shanghai Medical Journal

摘　　要：目的　研究肾上腺素预处理对大鼠离体心脏缺血再灌注损伤的保护作用 ,并探索其与剂量的关系。方法　大鼠随机分为 3组 ,每组 8只。A组 :即对照组 ,予静脉输注生理盐水 1ml/kg ,持续 3min ;B组 :静脉输注较低剂量肾上腺素 2 μg/kg ,持续 3min ;C组 :静脉输注较高剂量肾上腺素 2 0 μg/kg ,持续 3min。大鼠心脏稳定后 ,结扎冠状动脉左前降支 ,在Langendorff灌流模型下缺血 40min、再灌注 90min。 结果　B组缺血后心脏功能恢复良好 ,左心室压差为 ( 82 .96± 5 .60 )mmHg ,左室舒张末压为 ( 4.95± 0 .96)mmHg ,A组心脏功能恢复较差 ,左心室压差为 ( 70 .16± 5 .0 7)mmHg ,左室舒张末压为 ( 6.60± 1.0 4)mmHg ,B组较A组明显增高 (P <0 .0 5 ) ;B组灌注流出液中肌酸激酶值较低 ,B组梗死面积为 ( 2 8.70± 3 .41) % ,较A组的 ( 3 7.90± 6.2 9) %明显下降 (P <0 .0 5 )。Objective To determine whether epinephrine preconditioning exerts cardioprotective effects against ischemia/reperfusion injury in isolated rat heart, and to explore its dose-effect relationship with the concentration. Methods The rats were randomized divided into three groups(n=8 per group): (A) control group infused with 1 ml/kg saline for three minutes;(B) low concentration epinephrine group infused with 2 μg/kg epinephrine for three minutes;(C) high concentration epinephrine group infused with 20 μg/kg epinephrine for three minutes. After stabilization, the left anterior descending artery was ligated and the heart was subjected to 40 minutes of ischemia and 90 of minutes reperfusion according to Langendorff techniques.Results Group B had a better recovery in postischemic cardiac function at the end of reperfusion(LVDP and LVEDP were (82.96±5.60)mm Hg and (4.95±0.96)mm Hg versus (70.16±5.07)mm Hg and (6.60±1.04)mm Hg for group A respectively, P<0.05);Additionally, group B had a reduction in CK release and a limited size of infarct [(28.70±3.41)% versus (37.90±6.29)% for group A, P<0.05] .Conclusion Low concentration epinephrine preconditioning gives a cardioprotective effect on ischemic/reperfusion injury.

关 键 词：肾上腺素预处理 大鼠 离体心脏 缺血再灌注 心脏保护 

分 类 号：R541[医药卫生—心血管疾病]