利用油菜单倍体茎尖筛选抗菌核病变异体  

IN VITRO SELECTION OF RAPE VARIANTS RESISTANT TO OXALIC ACID USING HAPLOID STEM APEXES

作　　者：王亦菲[1] 黄剑华[1] 陆瑞菊[1] 孙月芳[1] 周润梅[1] 周志疆[2] 谢祝捷[2] 刘成洪[3] 

机构地区：[1]上海市农业遗传育种重点实验室上海市农业科学院生物技术研究中心 [2]上海市农业科学院园艺研究所,201106 [3]上海师范大学生命与环境科学学院,200234

出　　处：《核农学报》2002年第6期355-359,共5页Journal of Nuclear Agricultural Sciences

基　　金：上海市科技兴农攻关项目 (农科攻字 (98)第 0 1~ 13号 )

摘　　要：用平阳霉素 (Pingyangmycin)对甘蓝型油菜品系 9885和 9841的单倍体茎尖进行诱变处理 ,以草酸作为选择压力 ,获得一批耐草酸性明显比原始品种提高的变异体。经大田抗菌核病性鉴定 ,筛选出Mutagenic treatment was made of the haploid stem apexes rape strain ‘9841’ and ‘9885’ with Pingyangmycin.As a result of positive selection with oxalic acid providing selection pressure, variants with significantly higher tolerance to oxalic acid than the original ones were obtained.3 germplasm with significantly higher resistance to Sclerotinia sclerotiorum than cultivar Hu You 12 were selected from field test.

关 键 词：油菜 单倍体茎尖 筛选 抗菌核病变异体 平阳霉素 单倍体组织 草酸 抗病性 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]