移植肝热缺血损伤后糖原及酶组织化学活性的动态变化  

Dynamical changes of glycogen and enzyme-histochemistry activities of liver graft following warm ischemia injury in rat

作　　者：马毅[1] 何晓顺[1] 陈规划[1] 梁英杰[2] 胡瑞德[2] 黄洁夫[1] 

机构地区：[1]中山大学附属第一医院器官移植中心,广州510080 [2]中山大学中山医学院病理科

出　　处：《中华实验外科杂志》2003年第1期24-26,I001,共4页Chinese Journal of Experimental Surgery

基　　金：卫生部临床学科重点项目 (970 4 0 2 30 );广东省科委攻关项目 (99M 0 4 90 2G)

摘　　要：目的　探讨不同热缺血时间下大鼠移植肝组织糖原及酶组织化学活性变化规律 ,预测供肝耐受热缺血的安全时限。方法　用组织化学和细胞化学的方法 ,对大鼠移植肝组织进行动态观察。按热缺血时间随机分为 0、15、30、45、6 0min 5组 ,观察肝移植后的恢复性变化。结果　随热缺血时间的延长 ,肝组织琥珀酸脱氢酶 (SDH)、细胞色素氧化酶 (CO)和三磷酸腺苷酶 (Mg2 + ATPase)活性逐渐降低 ,糖原减少。热缺血 30min以前各组酶活性变化轻微 ,而 45、6 0min组均出现明显的酶活性降低和高碘酸 无色品红 (PAS)反应减低。 15、30min组 ,在复流后 2 4h糖原颗粒逐渐增多 ,呈明显的恢复 ,酶活性逐渐恢复至接近正常水平。 45、6 0min组 ,复流后 2 4hPAS反应和酶活性仍无明显恢复趋势。结论　移植肝热缺血在 30min以内 ,肝组织损伤仍处在可复性阶段 ,复流后能逐渐恢复至正常的形态和功能。移植肝糖原含量、酶组织化学活性的变化以及术后其恢复性的潜能可作为衡量供肝质量的重要标准。Objective To investigate the change patterns of glycogen and enzyme-histochemistry activities in rat liver graft under different warm ischemia time (WIT) and predict the tolerant time limitation of the liver graft to warm ischemia injury.Methods According to WIT,the rats were randomized into 5 groups with WIT being 0,15,30,45,60 min respectively,and liver graft specimens were subject to histochemical stain observation.The recovery changes of glycogen and enzyme-histochemistry activities were measured at 6,24 h following liver graft implantation.Results The activities of succinic dehydrogenase (SDH),cytochrome oxidase (CO),apyrase (Mg 2+-ATPase) and content of glycogen were gradually decreased after different WIT in a time-dependent manner,especially in the WIT 45 and WIT 60 groups.The activities of SDH,CO,Mg 2+-ATPase and content of glycogen of liver grafts within 30 min of warm ischemia were significantly recovered at 24 h after liver transplantation.The recovery potency of glycogen and enzyme-histochemistry activities of liver graft was less in 45 and 60 min groups.Conclusion Hepatic injury was reversible within 30 min of warm ischemia injury and could gradually resume normal morphologic structure and function after reperfusion.The activities of glycogen and enzyme histochemistry of liver graft and its recovery potency after reperfusion may serve as the important criteria to evaluate the quality of liver graft.

关 键 词：移植肝 糖原 酶 供肝 热缺血时间 安全时限 

分 类 号：R657.3[医药卫生—外科学]