胚胎干细胞在转化生长因子-β1诱导下表达vWF、CD34的研究  

Embryonic stem cells express vWF and CD34 induced by transferring growth factor-β1

作　　者：沈干[1] 吴春芳[1] 丛笑倩[1] 刘晓音[1] 刘伟[1] 曹谊林[1] 

机构地区：[1]上海第二医科大学附属第九人民医院整复外科组织工程实验室

出　　处：《中华实验外科杂志》2003年第1期49-50,I002,共3页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究发展规划 973项目 (1 9990 5430 4 )

摘　　要：目的　探讨胚胎干 (ES)细胞在转化生长因子 β1(TGF β1)诱导条件下的分化表现 ,为组织工程种子细胞来源提供诱导方法。方法　小鼠ES细胞 ,以 1× 10 - 9mol L维甲酸 (RA)和 3μg LTGF β1进行诱导分化。应用逆转录 聚合酶链反应 (RT PCR)及免疫组织化学验证细胞性质。结果　证实ES细胞经RA和TGF β1的诱导 ,3～ 4d出现圆形前体细胞 ,6d形成管状血管样结构。分化细胞可表达内皮细胞的标志vWF、CD34。结论　ES细胞在特定的条件下可分化为血管内皮细胞 ,有可能为构建组织工程化血管及其他人工血管的内皮化提供种子细胞来源。Objective To study the differentiation of embryonic stem (ES) cells under the culture condition of transferring growth factor-β1(TGF-β1),and find a inducing method for tissue engineering seed cells.Methods The mouse embryonic stem cells was induced to differentiate into endothelial cells by Retinoic acid(RA,1×10 -9mol/L) and 3 μg/L TGF-β1.Reverse transcription-polymerase chain reaction (RT-PCR),immunocytochemistry and scanning electron microscopy were used to analyze the morphology and type of the differentiated cells.Results After 3-4 days induction,many 'round' cells differentiated from ES cells,and formed tubular structures at 6th day,and these differentiated cells could express vWF and CD34.Conclusion ES cells can differentiate into endothelial cells,and form vessel-like structure under conditioned culture,which can provide sources of seed cells for tissue engineering blood vessel and other vascular prostheses.

关 键 词：胚胎干细胞 转化生长因子-Β1 内皮细胞 CD34 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]