高效液相色谱法测定小儿开胃口服液中异嗪皮啶含量  

Determination of isofraxidin in Xiaoerkaiwei Oral Liquid by HPLC

作　　者：于超[1] 凌康[2] 兰红梅[1] 王宇[1] 喻强[1] 

机构地区：[1]重庆市中药研究院药化室,重庆400063 [2]重庆医科大学附属一院,重庆400041

出　　处：《中成药》2002年第12期936-937,共2页Chinese Traditional Patent Medicine

摘　　要：目的 :建立用反相HPLC法测定小儿开胃口服液 (刺五加 ,鸡内金 ,草豆蔻 ,牵牛子 )中异嗪皮啶含量的方法。方法 :使用Symmetry C18色谱柱 (5 μm ,3.9× 15 0mm) ,以甲醇 - 0 .1%甲酸水溶液 (30∶70 )为流动相 ,流速 0 .8mL·min-1,检测波长为 342nm。柱温为 (30± 1)℃。结果 :在一定范围内异嗪皮啶含量呈现良好的线性关系 (r =0 .9993) ;精密度可靠 (RSD =1.87% ) ;样品在 6h内基本稳定。结论Objective: To establish a method for determination of isofraxidin in Xiaoerkaiwei Oral Liquid (Radix Acanthopanacis Senticosi, Endothelium Corneum Gigeriae Galli, Semen Alpiniae Katsumadai, Semen Pharbitidis, etc.) by RP HPLC. Methods: The HPLC system consisted of Symmetry C 18 column(3.9m×150mm, 5μm), methanol water (0.1% formic acid) (30∶70) mixture as a mobile phase, detection wavelength at 342nm, 0.8mL·min -1 of flow rate and column temperature at 30℃.Results: The calibration curves of the isofraxidin were linear ( r =0.9993). The precision were perfect ( RSD =1.87). The samples were stable in 6 h. The average recovery of Xiaoerkaiwei Oral Liquid was 102.4% with RSD of 1.76%.Conclusion: This method is simple, quick and specific, suitable for the quality control.

关 键 词：高效液相色谱法 小儿开胃口服液 异嗪皮啶 含量测定 质量控制 

分 类 号：R286.0[医药卫生—中药学]