利用PCR技术鉴别畜禽肉中禽源性成分研究  

Studies on the Identification of Poultry Origin Ingredients in Livestock and Poultry Meat by PCR Technique

作　　者：张晶鑫[1] 樊艳凤[1] 唐修君[1] 贾晓旭[1] 高玉时[1] 顾荣[1] 陆俊贤[1] 王珏[1] 

机构地区：[1]中国农业科学院家禽研究所,江苏扬州225125

出　　处：《湖北农业科学》2016年第15期4021-4023,共3页Hubei Agricultural Sciences

基　　金：扬州市社会发展前瞻性研究项目(YZ2014188);国家自然科学基金项目(31372277)

摘　　要：为了建立一种快速、特异的禽源性成分检测方法,以线粒体16S r RNA基因序列为靶位点设计鸡、鸽、鹌鹑特异性引物,以常见畜禽肉(包括羊肉、牛肉、猪肉、兔肉、鸽肉、鹌鹑肉、鸡肉、鸭肉、鹅肉等)DNA为模板,进行PCR扩增和特异性检测。结果表明,筛选的引物能够有效地对动物源性成分进行检测,方便简洁,可快速鉴别畜禽肉食品中含有的鸡源性、鸽源性、鹌鹑源性成分。In order to establish a quick and specific peculiar method, which could identify the components of poultry origin.16 S r RNA gene sequence was use as target site, the specific primers of chicken, pigeon meat and quail meat were designed.The DNA of common livestock and poultry meat including mutton, beef, pork, rabbit meat, pigeon meat, quail meat,chicken, duck and goose were used as template. Though PCR amplification and specific detection, a quick determination method was established to identify the components of poultry origin. The results showed that the selected primer could identify the components of animal origin effectively and quickly. The method was convenient and concise, and could detect the chicken origin, pigeon origin and quail origin in poultry food quickly and accurately.

关 键 词：禽源性成分 16S r RNA基因 PCR 鉴别 

分 类 号：TS251.5[轻工技术与工程—农产品加工及贮藏工程]