安氟醚麻醉大鼠不同时期脑Na^+,K^+-ATP酶活性变化  

Anaesthesia of enflurane on Na^+,K^+-ATPase activity change at different stage in rat brain

作　　者：李元涛[1] 戴体俊[2] 曾因明[1] 刘菊英[1] 

机构地区：[1]郧阳医学院麻醉学系,十堰442000 [2]徐州医学院江苏省麻醉学重点实验室,徐州221002

出　　处：《中国药理学通报》2002年第6期649-651,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题No 39970 715;江苏省自然科学基金资助课题 NoBK2 0 0 114 3

摘　　要：目的　动态观察安氟醚吸入麻醉大鼠不同时期各脑区Na+ ,K+ ATP酶活性变化 ,探讨安氟醚麻醉作用机制。方法　 40只SD雌性大鼠按麻醉不同时期随机均分为 5组 :对照组、诱导期组、麻醉期组、恢复期组和清醒期组。用分光光度法测定不同时期大脑皮层、脑干、海马Na+ ,K+ ATP酶活性。结果　与对照组比较 ,大鼠大脑皮层、脑干、海马Na+ ,K+ ATP酶活性在诱导期即开始下降 ,其中大脑皮层和海马酶活性降低有统计学意义 (P <0 0 5 ) ;麻醉期Na+ ,K+ ATP酶活性下降至最低 (P <0 0 1) ;恢复期酶活性又开始回升 ,但仍低于对照组 (P <0 0 5 ,P <0 0 1) ;清醒期基本恢复至正常水平 (P >0 0 5 )。且麻醉深度变化与Na+ ,K+ ATP酶活性抑制相关。结论　安氟醚可明显抑制Na+ ,K+ ATP酶活性 ,且与行为变化相平行 ,提示安氟醚通过抑制Na+ ,K+ ATP酶活性而发挥麻醉效应 。AIM To observe dynamicly the effects of enflurane on the Na +,K + ATPase activity in different rat brain region at different stage and explore the anaesthetic mechanism of enflurane. METHODS Forty femal rats were divided randomly into five groups as follows: control group; induction group; anaesthetic group; recovery group and awake group. The Na +,K + ATPase activity at different stage in cerebral cortex, brain stem and hippocampus were measured by spectroscopic analysis. RESULTS The activity of Na +,K + ATPase in cerebral cortex,brain stem and hippocampus begun to reduce during the induction stage, especially, the activity of Na +,K + ATPase in cerebral cortex and hippocampus reduced significantly( P <0\^05); it reduced to the lowest during anaesthetic stage and begun to increase during recovery stage but still lower than it in control group( P<0 05,P<0 01) ; during awake stage, it almost recovered to nomal level( P >0 05). Also, the changes of the depth of anesthesia were related to the inhibition of Na +,K + ATPase activity.CONCLUSION Enflurane significantly inhibited the activity of Na +,K + ATPase and it paralled to behavioural changes, which suggested enflurane worked through inhibiting the activity of Na +,K + ATPase, the depth of anaesthesia was related to the degree of inhibition of the Na +,K + ATPase activity.

关 键 词：安氟醚 麻醉 NA^+ K^+-ATP酶 麻醉机制 

分 类 号：R614[医药卫生—麻醉学] R965[医药卫生—外科学]