氧化苦参碱诱导卵巢癌SKOV3细胞凋亡作用的实验研究  

Induction of human ovarian cancer SKOV3 cells apoptosis by oxymatrine

作　　者：侯华新[1] 黎丹戎[2] 栾英姿[2] 陈丽[3] 

机构地区：[1]广西医科大学化学教研室,南宁530021 [2]广西肿瘤防治研究所,南宁530021 [3]广西医科大学,2000级研究生南宁530021

出　　处：《中国药理学通报》2002年第6期704-707,共4页Chinese Pharmacological Bulletin

基　　金：广西自然科学基金资助课题 No桂科自 0 135 0 30

摘　　要：目的　探讨氧化苦参碱对卵巢癌细胞SKOV3诱导凋亡作用。方法　采用MTT法、DNA凝胶电泳、生长曲线、荧光染色等实验技术研究氧化苦参碱在体外对SKOV3细胞的增殖抑制和诱导细胞凋亡的作用。结果　氧化苦参碱对SKOV3细胞生长有明显的抑制作用 ,且这种抑制作用呈浓度依赖性。细胞经 0 189mmol·L-1和 0 3 78mmol·L-1的氧化苦参碱作用 48h后 ,即呈现典型的细胞凋亡形态学变化和DNA片段的梯形条带。AIM To observe the effects of Oxymatrine on induction of apoptosis in human ovarian cancer SKOV3 cells. METHODS Apoptosis induced by Oxymatrine in human ovarian cancer SKOV3 cells was tested by MTT assay, fluorescent microscope, and DNA gel eletrophoresis. RESULTS SKOV3 cell viability dropped down depending on the Oxymatrine concentration and treatment time. When incubated with Oxymatrine (0 189 mmol·L -1 and 0 378 mmol·L -1 ) for 48 h, SKOV3 cells showed morphological changes associated with the characters of apoptosis under fluorescent microscope. Typical DNA ladder was found during gel eletrophoresis. CONCLUSION Oxymatrine can induce apoptosis in SKOV3 cell lines.

关 键 词：氧化苦参碱 卵巢癌 细胞凋亡 苦参 MTT法 DNA凝胶电泳 

分 类 号：R273.731[医药卫生—中西医结合] R28[医药卫生—中医肿瘤科]