白果内酯治疗实验大鼠肺孢子虫肺炎电镜观察  

The anti-Pneumocystis carinii effect of bilobalide in the animal model with transmission electron microscope

作　　者：倪小毅[1] 唐小葵[1] 陈雅棠[1] 王健[2] 廖晓刚[3] 

机构地区：[1]重庆医科大学附一院传染病寄生虫病研究所 [2]深圳市第二人民医院肺科 [3]重庆医科大学电镜室

出　　处：《中国人兽共患病杂志》2003年第1期9-12,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助 (No :3 0 0 70 680 );重庆市卫生局青年科学基金 99-3 0 19

摘　　要：目的　观察白果内酯对卡氏肺孢子虫超微结构的影响。方法　地塞米松连续皮下注射Wistar大鼠 6周 ,建立大鼠卡氏肺孢子虫肺炎动物模型 ,腹腔内注射白果内酯 30mg/(kg·d)× 8d。停药 1周后取大鼠肺组织作超薄切片 ,透射电镜观察。结果　白果内酯作用后的卡氏肺孢子虫虫体有大量空泡形成 ,细胞器肿胀破坏 ,髓样结构形成 ,胞膜破坏 ,胞质内出现电子密度较高颗粒。结论　白果内酯可破坏卡氏肺孢子虫的超微结构 ,从而引起虫体死亡。Aim To study the ultrastructural changes of Pneumocystis carinii Pneumonia(PCP)treated with bilobalide in the animal model Methods PCP animal models were established by hypodermic injection of dexamethasone in rats,twice a week for as long as 6 weeks Bilobalide was administered intraperitoneally at dose of 30mg/kg/d for 8 days Utrastructural observation was taken on rats lung tissues after stopping treatment for a week with transmission electron microscope Results There were much vacuoles formation,swollen organelles,medullary formation,broken cell membrane and some granules with higher electron density in Pneumocystis carinii after treatment with bilobalide Conclusion Bilobalide could destroy the ultrastructures of Pneumocystis carinii resulting in the death of the parasites

关 键 词：电镜观察 卡氏肺孢子虫 卡氏肺孢子虫肺炎 白果内醌 超微结构 

分 类 号：R563.1[医药卫生—呼吸系统]