低密度脂蛋白诱导巨噬细胞髓过氧化物酶活性的研究  

Study of the relation between low density lipoprotein and macrophage-myeloperoxidase

作　　者：武军驻[1] 洪嘉玲[1] 

机构地区：[1]武汉市武汉大学医学院生化教研室,武汉430071

出　　处：《中国免疫学杂志》2003年第1期16-18,共3页Chinese Journal of Immunology

摘　　要：目的 :研究低密度脂蛋白 (LDL)与巨噬细胞髓过氧化物酶 (MPO)活性的关系。方法 :LDL诱导培养的小鼠腹水巨噬细胞 ,用MPO催化底物邻联二茴香胺氧化的方法测定MPO活性 ;以逆转录多聚酶链反应 (RT PCR)的方法检测MPO基因的表达 ,借此观察LDL与MPO活性的关系。结果 :LDL可以促进巨噬细胞内及分泌的髓过氧化物酶活性升高 ,但远比细菌脂多糖 (LPS)的作用小 ;氧化型低密度脂蛋白 (OX LDL)没有此作用 ;随着LDL作用时间的延长巨噬细胞内及分泌的髓过氧化物酶活性逐渐升高 ,但随着LDL浓度的增加髓过氧化物酶基因的表达达到最大值后便维持该水平而不再随LDL浓度的变化而变化。结论 :LDL非特异性地诱导巨噬细胞MPO活性升高以及分泌增强 ,加强自身的氧化吸收 ;MPO活性升高的原因可能主要为巨噬细胞内酶活性的激活及分泌增强而非基因表达的增强。Objective:To study the relation between low density lipoprotein and macrophage myeloperoxidase.Methods:Using the reaction that MPO catalyze the oxidation of o dianisidin hydrochloride,MPO activity was determined.Using reverse transcription polymerase chain reaction(RT PCR),MPO gene expression was determined.The two methods were used to observe the relationship between low density lipoprotein and macrophage myeloperoxidase activity.Results:LDL was able to accelerate the hoist of MPO activity, but its effect is less than LPS OX LDL have no this effect When the time of LDL effect was prolonged, MPO activity was enhancing little by little When the concentration of LDL effect was increased, MPO activity was enhancing to max little by little, then it come to plateau Conclusion:LDL non especially induced the hoist of MPO activity and the swelling of MPO secretion The reason of the hoist of MPO activity was likely to make MPO into action and enhance its secretion, but not MPO gene expression

关 键 词：髓过氧化物酶 低密度脂蛋白 巨噬细胞 逆转录多聚酶链反应 

分 类 号：Q513.5[生物学—生物化学]