针刺对备用背根节神经元TrkC表达的影响  

The TrkC Expression in Spared Dorsal Root Ganglion Following Acupuncture

作　　者：王廷华[1] 柯青[1] 吴林艳[1] 张晓[1] 

机构地区：[1]昆明医学院神经科学研究所,昆明650031

出　　处：《四川大学学报（医学版）》2003年第1期40-42,共3页Journal of Sichuan University(Medical Sciences)

摘　　要：目的　探讨针刺对备用背根节 (DRG) Trk C表达的影响。方法　将成年雄猫分为三组 ,即假手术组、单侧部分背根切除术组、单侧部分背根切除术并针刺组。通过免疫组化 ABC法 ,观察各组 L6 DRG中 Trk C免疫反应 (Trk C- IR)神经元数量。结果　在假手术组 Trk C- IR主要定位于 L6 DRG的大神经元及少数中小神经元胞浆内 ;部分背根切断后 ,Trk C- IR大神经元数量明显减少 ,而中小神经元数量则明显增多 (P<0 .0 5 ) ;针刺后 ,Trk C- IR大神经元数量较手术组又明显增多 ,且反应强度明显增加 (P<0 .0 5 ) ,而对中小神经元 Trk C阳性细胞数无明显影响。结论　针刺促进 Trk C在大神经元的表达上调 ,提示 TrkObjective Investigating the TrkC expression in the spared dorsal root ganglion (DRG) after acupuncture stimulation of spared root. Methods Fifteen male adult cats were divided into three groups. Five cats were in the sham operation group; another five cats were subjected to unilateral root rhizotomy with (L 1 L 5,L 7 S 2 DRG sectioned, L 6 DRG spared) and lived 7 days after operation; the last five cats were placed under electro needle stimuli alternative at two groups of acupoints〔including Tsusanli (St.36) and Hsüanchung (G.B.39), Fut'u(femur)(St.32) and Sanyinchiao (Sp.6) located in the distribution area of spinal nerve L 6 on the operation side〕 30 min a day for 7 days after unilateral root rhizotomy. On the 7th day, all animals were sacrificed. The L 6 DRG from the experimental side of each animal was taken and made into frozen sections 20μm in thickness. The sections were stained under the same condition using specific TrkC antibody(1∶1000, Santa)by immunohistochemistry ABC method. The number of TrkC immunoreaction (IR) neurons of DRG was observed and measured. Results In L 6 DRG of sham operated group, TrkC IR was found mainly distributed in the cell plasm of some large sized neurons and a few medium and small sized neurons. Following partial dorsal root rhizotomy ,the number of trKC IR large sized neurons apparently decreased, while the number of the small and medium sized neurons markedly increased ( P<0 05) . The number and reaction level of TrkC IR large sized neurons apparently increased after acupuncture( P<0 05) , but the number of TrkC IR small and medium sized neurons were not significantly changed.Conclusion This experimental study demonstrates that TrkC expression in L 6 DRG neurons is upregulated after the acupuncture stimulation of spared root, suggesting that TrkC may be related to spinal plasticity.

关 键 词：TrkC表达 背根节 部分去背根 针刺 

分 类 号：R245[医药卫生—针灸推拿学]