四环素-哌嗪雌酚酮对小鼠骺板Ⅰ型胶原表达的影响  

Effects of XW630 on the Expression of Type Ⅰ Collagen Protein in the Epiphyseal Plates of Mice

作　　者：李灵芝[1] 翁玲玲[1] 张永亮 楼荣良 郑虎 

机构地区：[1]四川大学华西药学院药物化学教研室,成都610041

出　　处：《四川大学学报（医学版）》2003年第1期86-88,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金重点项目资助 (批准号 3 943 0 12 0 )

摘　　要：目的　研究四环素 -哌嗪雌酚酮 (XW6 30 )对胚胎小鼠骺板 型胶原的表达的影响。方法　采用胚胎小鼠体外培养模型 ,通过免疫组织化学手段 ,观察 XW6 30对 型胶原蛋白表达水平的影响。结果　当培养基中XW6 30浓度为 10 - 7m ol/L和 10 - 8mol/L时 ,各区 型胶原蛋白免疫组织化学染色阳性细胞面积与对照组相比均显著增加 ;与同浓度的雌酚酮组相比 ,10 - 7mol/L XW6 30组增殖区和肥大区阳性细胞面积增加。当浓度降至 10 - 9mol/L时 ,只有肥大区阳性细胞面积增加。随浓度升高 ,各区阳性细胞面积呈增加趋势。结论　在体外培养条件下 ,XW6 30剂量依赖地上调小鼠骺板静止区、增殖区和肥大区 型胶原蛋白的表达水平。Objective To identify the effect of XW630 on the expression of type Ⅰ collagen in the epiphyseal plates of mice. Methods The immunohistochemical method was used to measure the change of type Ⅰ collagen protein after being treated with XW630. Results With 10 -7 mol/L or 10 -8 mol/L of XW630, the area of positive stain cells in every zones increased significantly compared to the control group. A more significant increase of the area of positive cells in the proliferative zone and hypertrophic zone was observed in the XW630 group than in the estrone group at a concentration of 10 -7 mol/L. When the XW630 was decreased to 10 -9 mol/L, the increase of positive cells was only found in the hypertrophic zone. The area of positive cells increased with the concentration of XW630. Conclusion The XW630 upregulates the dose dependent expression of type Ⅰ collagen protein in the resting zone, proliferative zone, and hypertrophic zone in the epiphyseal plates of mice in vitro.

关 键 词：骺板 I型胶原 四环素-哌嗪雌酚酮 免疫组织化学 

分 类 号：R965[医药卫生—药理学]