通脉冲剂预防动脉粥样硬化闭塞症的实验研究  

Experimental Study on Prophylaxis of Atherosclerosis Obliterans with Tongmai Granules

作　　者：刘健[1] 齐清会[1] 

机构地区：[1]天津医科大学总医院,天津300052

出　　处：《中国中西医结合外科杂志》2002年第6期424-427,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

摘　　要：目的 :利用祖国传统医学的活血化瘀原理 ,结合现代医学理论 ,从保护内皮细胞和拮抗氧自由基损伤的角度探求预防动脉粥样硬化闭塞症 (atherosclerosisobliterans ,ASO)的有效方案。 方法 :选择健康雄性日本杂交大耳白家兔 2 0只 ,随机分为预防组和对照组 ,均用气囊导管损伤动脉内膜、免疫损伤、高胆固醇饲料喂养的方法诱导制作ASO动物模型。在制模的同时 ,预防组给予通脉冲剂治疗 ,对照组不给予药物治疗 ,共 2 0周。实验过程中动态观察家兔跛行情况及总甘油三脂 (totaltriglyceride ,TG)、总胆固醇 (totalcholesterol,TC)、血内皮素 (endothelin ,ET)、一氧化氮 (nitricoxide ,NO)、超氧化物歧化酶 (superoxidedismutaseSOD)及脂质过氧化物 (lipidperoxide ,LPO)的变化。实验结束后取髂股动脉做病理检查。 结果 :预防组仅 1只家兔出现跛行情况 ,TC、TG、ET、LPO升高和NO、SOD降低的程度明显低于对照组。与对照组相比 ,预防组动脉班块的面积较小 ,光镜下改变也较轻微。 结论 :通脉冲剂具有降低血脂、血胆固醇 ,拮抗ET、氧自由基和增加NO水平的作用 ,对于预防ASO的形成有很好的效果。Objective:To explore effective methods to prevent atherosclerosis obliterans(ASO)by protecting endothelical cell and resisting the injury of oxygen free radicals according to the theory of Huoxuehuayu(activating blood circulation and dispersing stasis of TCM)and modem medicine Methods:Twenty healthy male Japanese rabbits were divided into prophylactic and control groups.The ASO model was made with methods of mechanical injury,immune injury,and high cholesterol fodder.Tongmai granules were given to the prophylactic group but not to the control.Lameness was observed and levels of total triglyceride(TG),total cholesterol(TC),superoxide dismutase(SOD),lipid peroxide(LPO),endothelin(ET)and nitric oxide(NO)in serum were measured at the end of the experiment,iliofemoral arteries were extracted for pathologic examination.Results:Only one lameness was observed in the prophylactic group.The degree of increase of TC,TG,ET,LPO,and decrease of NO and SOD were lower in the prophylactic group than in the control group obviously.The phathologic changes of ASO shown under light microscopy were also milder than those in control group.Conclution:Tongmai granules has the effects of decreasing TC and TG,antagonizing ET and oxygen free radicals and increasing the level of NO,thus may prevent the formation of ASO.

关 键 词：通脉冲剂 动脉粥样硬化闭塞症 预防 一氧化氮 内皮素 氧自由基 中医药疗法 

分 类 号：R259.435[医药卫生—中西医结合]