蛋白质在离子交换介质中的动态吸附性能  

Dynamic Adsorption Performance of Proteins to CM Sepharose FF Adsorbents

作　　者：姚善泾[1] 关怡新[1] 俞丽华[1] 

机构地区：[1]浙江大学化学工程与生物工程学系,浙江杭州310027

出　　处：《高校化学工程学报》2002年第6期663-669,共7页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金资助项目(20076042)。

摘　　要：研究了离子交换介质 CM-Sepharose FF 对几种不同的蛋白质——溶菌酶、木瓜蛋白酶、牛血清蛋白的动态吸附性能,考察了pH值、溶液粘度、初始蛋白浓度及吸附剂量等因素的影响。结果表明,这些参数会对吸附剂的动态吸附性能产生不同程度的影响,而这些蛋白质也都显示了相似的规律。采用两相阻力模型描述了蛋白质在离子交换介质的动态吸附性能,计算得到了蛋白质在吸附剂颗粒中的扩散系数,有助于进一步研究蛋白质吸附过程的传质现象。The protein adsorption at liquid-solid interfaces is of great importance because the understanding of interaction between proteins and ion exchange resin will facilitate the purification of proteins and the scale-up of the process. Adsorption kinetics were studied for the binding of proteins to CM Sepharose FF. The influences of temperature, pH, viscosity, initial concentration and the volume of adsorbents on the adsorption characteristics were investigated in detail. The two-phase resistance model describing the dynamic adsorption process of lysozyme, papain, BSA to CM Sepharose FF was presented. Using this model and the dynamic adsorption data the pore diffusion coefficients were determined. Comparing with the experimental results, the success of the presented theoretical model is apparent, and it shows that the model can be used to predict the influence of many operation parameters on the performance of ion exchange separation.

关 键 词：蛋白质 离子交换介质 动态吸附性能 扩散系数 溶菌酶 分离 

分 类 号：TQ28[化学工程—有机化工] TQ028.3