测定切花中过氧化氢酶活性的3种常用方法的比较  

Comparison of Three Different Methods of Determining Catalase Activities in Cut Flowers

作　　者：陈晓敏[1] 

机构地区：[1]华南热带农业大学园艺学院,海南儋州571737

出　　处：《热带农业科学》2002年第5期13-16,共4页Chinese Journal of Tropical Agriculture

摘　　要：比较了切花中过氧化氢酶(CAT)的2种提取方法和3种检测方法。结果表明:提取CAT时,缓冲剂中不需要加入过多的保护剂;采用直接紫外分光光度法检测CAT活性,其灵敏度、准确度都优于碘量滴定法和紫外分光光度碘量方法,因而适用于植物CAT活性的大量样品快速测定。The methods of extraction and activity determination of catalase from cut Phalaenopsis amabilis were compared.The results showed that too much protective substance was not necessary in the buffer during catalase extraction.The direct ultraviolet spectrophotometric method of determination of catalase activity was more sensitive and precise than the iodine titra tion method and the ultraviolet spectrophotometric iodine method.Thus it is suitable for fast determination of catalase activities in plants.

关 键 词：切花 过氧化氢酶 酶活性 测定方法 碘量滴定法 紫外分光光度碘量法 直接紫外分光光度法 

分 类 号：S68[农业科学—观赏园艺] Q554.6[农业科学—园艺学]