大腹园蛛丝腺蛋白二级结构的圆二色性研究  

CD Spectrums of Ampullate Protein conformation of Araneus Ventricosus Spiders

作　　者：盛家镛[1] 许箐[1] 刘敏[1] 潘志娟[1] 陈宇岳[1] 

机构地区：[1]苏州大学材料工程学院,苏州215021

出　　处：《科学技术与工程》2002年第6期27-30,共4页Science Technology and Engineering

基　　金：国家自然科学基金(50173018)资助

摘　　要：用圆二色光谱研究了大腹园蛛各丝腺蛋白的分子构象、大、小囊状腺体蛋白的分子构象以无规卷曲和β-折替结构为主;而其管状腺、集合状腺、鞭毛状腺蛋白的 CD 谱均显示出有α-螺旋结构的特征。用计算法所得这五种丝腺体蛋白的二级结构中含有6.2%～13.9%不等的β-转角,这对蛛丝蛋白形成独特的结构起着重要作用。还研究了放置时间对这五种丝腺体蛋白构象的影响。Gland proteins' molecular conformations of Araneus ventricosus spiders were studied with CD spectrums.The protein molecular conformations of major and minor ampullate glands were basically random coil and β-sheet.The protein conformations of tubulformes,aggregatae and falgelliform glands had the α-helix characteristics.There were 6.2%～13.9% β-turn structures in these proteins,which played important roles in the spider silk structures.The effects of time on the conformations were investigated also.

关 键 词：蜘蛛丝 蛋白质 大腹园蛛 丝腺蛋白 圆二色性 

分 类 号：Q959.226[生物学—动物学]