司帕沙星在BTR缓冲溶液中的紫外光谱性质研究与应用  

Study on Ultraviolet Spectrum Property of Sparfloxacin Acid Media and its Application

作　　者：曹玺珉[1] 杜黎明[1] 

机构地区：[1]山西师范大学分析测试中心,山西临汾041004

出　　处：《山西师范大学学报（自然科学版）》2003年第1期63-65,共3页Journal of Shanxi Normal University(Natural Science Edition)

基　　金：山西省自然科学基金资助项目 (编号 :2 0 0 1 1 0 1 6) .

摘　　要：研究了不同pH介质中司帕沙星的紫外光谱性质 ,发现pH3.0的BTR缓冲液为紫外分光光度法测定司帕沙星的最佳条件 .在吸收波长 2 98nm处 ,其表观摩尔吸光系数为 2 .62×1 0 4 Lmol- 1 cm- 1 ,司帕沙星在 1 .8mg/L3.0mg/L浓度范围内符合比尔定律 ,其回收率为 1 0 0 .7% ,相对标准偏差为 0 .2 3% .A new ultraviolet spectrophotometry method for the determination of sparfloxacin (SPFX) has been developed based on 0.04mol/L boric acid phosphoric acid acetic acid buffer solution, the best condition in detecting sparfloxacin is proposed. The sparfloxacin ultraviolet spectrum property has been studied in different pH media. In Britton Robinson buffer solution, the ultraviolet intensity reached its maximum at pH value of 3.0. The solutions obeyed Beer′s law in the concentration range of 1.8mg/L^30mg/L at 298.3nm, ε =2.62×10 4L/mol cm. The results obtained by the proposed methods are in good agreement with the determination of sparfloxacin in tablets, the average recovery and RSD were 100.7% and 0.23% respectively.

关 键 词：紫外光谱 司帕沙星 BTR缓冲溶液 紫外分光光度法 抗菌素 含量测定 

分 类 号：R978.1[医药卫生—药品] R927.2[医药卫生—药学]