红霉素肟的HPLC分析  

The HPLC Analysis of Erythromycin 9-Oxime

作　　者：王静宇[1] 罗爱芹[1] 邓玉林[1] 顾峻岭[1] 马文[1] 

机构地区：[1]北京理工大学化工与材料学院,北京100081

出　　处：《北京理工大学学报》2002年第6期771-773,共3页Transactions of Beijing Institute of Technology

基　　金：国家自然科学基金资助项目(K29775006)

摘　　要：用HPLC方法分析合成甲基红霉素中间体红霉素肟的含量,考察了该方法的稳定性.采用外标法,色谱条件:色谱柱Nova-pakC18;流动相V(CH3CN)∶V(0.033mol/LKH2PO4)=30∶70,检测波长205nm,检测灵敏度0.1AUFS.结果显示该方法有很好的稳定性和精密度.High performance liquid chromatography (HPLC) has been widely used in the quantitative analysis of erythromycin 9oxime, an important intermediate for the synthesis of new erythromycin derivatives. HPLC method is used to analyse the content of erythromycin 9oxime, and the stability of the method is evaluated. The results show that this method has a good stability and high accuracy. External method is applied, with the following chromatographic conditions: description of column: Novapak C18, eluent used: V(CH3CN)∶V(0033 mol/L KH2PO4)=(30∶70), detection wavelength: 205 nm, detection sensitity 01AUFS.

关 键 词：HPLC分析 高效液相色谱法 红霉素肟 定量分析 稳定性 药物中间体 含量测定 

分 类 号：TQ465.5[化学工程—制药化工] TQ46