PHO80、PHO85基因和芽殖酵母金属离子耐受  

Involvement of PHO80 and PHO85 Genes in Saccharomyces cerevisiae Ion Tolerance

作　　者：毛西成[1] 夏宇蕾[1] 胡亚芳 陆长德[1] 

机构地区：[1]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031

出　　处：《生物化学与生物物理学报》2003年第1期86-91,共6页

基　　金：国家自然科学基金资助项目 (No .396 30 16 0 )~~

摘　　要：PHO8 5基因是芽殖酵母中的一个多功能基因。它参与了无机磷酸的代谢、碳源利用、糖原积累、特定蛋白质的降解和细胞周期调控。研究了酵母株YPH499及其衍生的pho85缺失株、pho80缺失株、pap1(pcl7)缺失株在不同浓度的不同金属离子中的存活情况 ,结果表明和芽殖酵母YPH499相比 ,pho85缺失株和pho80缺失株表现出对K+ 、Mg2 + 、Zn2 + 、Ca2 + 和Mn2 + 的耐受下降 ,而PAP1基因的缺失则不会导致芽殖酵母对上述金属离子的敏感性的变化 ;而对Cu2 + ,3株突变株都表现出和YPH499相同的耐受性。同时测定了各缺失株和YPH499对上述金属离子的半致死浓度以及pho85缺失株、pho80缺失株和YPH499的细胞内总钙量。这些结果显示 ,PHO85蛋白激酶通过和它的PCLPHO80而不是PAP1结合 ,参与了芽殖酵母K+ 、Mg2 + 、Zn2 + 、Ca2 + 和Mn2 + 离子平衡的调控。PHO85和PHO80基因的缺失损害了芽殖酵母钙的储存。PHO85 is a versatile gene in Saccharomyces cerevisiae, which is involved in metabolism of inorganic phosphate and usage of carbon source, accumulation of glycogen, regulation of protein stability and cell cycle control. The viability of wild type budding yeast strain YPH499 and its derivative pho85Δ mutant, pho80Δ mutant, and pap1(pcl7)Δ mutant in different cations were investigated and their tolerance to the cations(LC 50) was measured. The results showed that the deletion of PHO85 or PHO80 gene both increased sensibility of Sacchromyces cerevisiae to ions K+, Mg 2+, Zn 2+, Ca 2+ and Mn 2+, while the deletion of pap1(pcl7) gene did not lead to such phenotype. The difference between the patterns of relative growth curve of the mutants and wild type strain in the above ions also implied that PHO80 was the unique PCLs in complex with PHO85 CDK, that were contributed to K+ and Mg 2+ ion homeostasis control and there were some other PCLs besides PHO80 that were involved in Zn 2+, Ca 2+ and Mn 2+ tolerance regulation as cyclin of PHO85 CDK. Furthermore, the amount of the total cellular calcium of pho85Δ mutant, pho80Δ mutant and YPH499 indicated that the ability of calcium accumulation of pho85Δ mutant and pho80Δ mutant was impaired.

关 键 词：基因缺失株 PHO80 PHO85 芽殖酵母 金属离子耐受 

分 类 号：Q781[生物学—分子生物学]