鹿茸多肽的分离纯化及药理活性  

Research on the Purification and Characterization of Polypeptide from Velvet Antler

作　　者：王丰[1] 梅子青[1] 周秋丽[2] 王本祥[3] 

机构地区：[1]吉林大学生命科学学院,长春130023 [2]吉林大学生物工程研究所,长春130021 [3]长春中医学院附属医院新药研究中心,长春130021

出　　处：《吉林大学学报（理学版）》2003年第1期111-114,共4页Journal of Jilin University:Science Edition

摘　　要：采用凝胶过滤层析、离子交换层析以及高效液相方法,从马鹿鲜鹿茸分离得到了一种多肽,其在SDS-PAGE电泳上显示为一条带,HPLC图谱为单峰,质谱显示其相对分子量为3095.1.氨基酸组成分析结果表明,此多肽主要含有缬氨酸、丙氨酸、赖氨酸和甘氨酸,没有半胱氨酸.经FDNB法测定,其N-末端氨基酸为缬氨酸.活性检测表明,该多肽能够明显促进表皮细胞和BRL肝细胞株的增殖.The velvet antler polypeptide was extracted and purified by gel filtration, ion exchange chromatography and HPLC, which showed a single peak in HPLC chromatography and a single band in SDSPAGE. The molecular weight measured by MALDI/TOF/MS spectrum is 3 095.1. The polypeptide consists mostly of Ala, Phe, Lys, Gly, but no Cys. The Nterminal amino acid of the polypeptide was detected by FDNB method to be Val. The experiment of pharmaceutical activity indicated that the polypeptide could obviously stimulate proliferation of the epithelial cells and BRL cells. 

关 键 词：鹿茸 多肽 分离 纯化 药理活性 中药 理化性质 凝胶过滤层析 离子交换层析 

分 类 号：R284.2[医药卫生—中药学] R285[医药卫生—中医学]