Preimplantation gender diagnosis by fluorescence in situ hybridization  

应用荧光原位杂交技术进行胚胎植入前性别诊断(英文)

作　　者：徐艳文[1] 庄广伦[1] 舒益民[1] 李满[1] 周灿权[1] 张敏芳[1] 

机构地区：[1]中山医科大学附属第一医院妇产科生殖医学研究中心,广州510080

出　　处：《Chinese Medical Journal》2002年第6期874-877,152-153,共4页中华医学杂志（英文版）

基　　金：adoctoralgrantfromtheNationalEducationalCommitteeofChina (No 9948)

摘　　要：Objective To describe the clinical application of fluorescence in situ hybridization (FISH) in preimplantation gender diagnosis.Methods Preimplantation gender diagnosis was performed in 2 female hemophilia A carriers, 1 male patient with glucose-6-phosphate dehydrogenase (G-6-PD) deficiency and 2 male patients with Y chromosome abnormality. Embryo sex was identified by FISH in total of 6 treatment cycles.Results A total of 123 cumulus-oocytes were retrieved in 6 treatment cycles. Sixty-one embryos were available for embryo biopsy. The success rate of biopsy was 86.9% (53/61), with a further cleavage rate of 62.3% (33/53). In the FISH procedure, one cell was lost during fixation, leading to a 98.1% (52/53) fixation rate. Totally, 16 female embryos and 3 male embryos were transferred to 5 patients in 6 cycles. Three healthy babies were born. The diagnosis was confirmed by subsequent analysis of amniocytes and embryonic buds after embryo reduction.Conclusions FISH is an efficient and reliable technique for determining the sex of human preimplantation embryos. Selective abortion and births of affected children can be avoided by preimplantation gender diagnosis.OBJECTIVE: To describe the clinical application of fluorescence in situ hybridization (FISH ) in preimplantation gender diagnosis. METHODS: Preimplantation gender diagnosis was performed in 2 female hemophilia A carriers, 1 male patient with glucose-6-phosphate dehydrogenase (G-6-PD) deficiency and 2 male patients with Y chromosome abnormality. Embryo sex was identified by FISH in total of 6 treatment cycles. RESULTS: A total of 123 cumulus-oocytes were retrieved in 6 treatment cycles. Sixty-one embryos were available for embryo biopsy. The success rate of biopsy was 86.9% (53/61), with a further cleavage rate of 62.3% (33/53). In the FISH procedure, one cell was lost during fixation, leading to a 98.1% (52/53) fixation rate. Totally, 16 female embryos and 3 male embryos were transferred to 5 patients in 6 cycles. Three healthy babies were born. The diagnosis was confirmed by subsequent analysis of amniocytes and embryonic buds after embryo reduction. CONCLUSIONS: FISH is an efficient and reliable technique for determining the sex of human preimplantation embryos.Selective abortion and births of affected children can be avoided by preimplantation gender diagnosis.

关 键 词：BLASTOCYST In Situ Hybridization  Fluorescence Sex Determination (Analysis) Adult AMNIOCENTESIS Biopsy Female Humans Male Pregnancy Research Support  Non-U.S. Gov't 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]