Vascular endothelial growth factor gene transfer improves host endothelialization of xenogeneic biologic heart valve in vivo  

血管内皮生长因子基因转移促进异种生物瓣膜宿主内皮化的研究(英文)

作　　者：张中明[1] 董红燕[2] 刘金东[3] 王伟[1] 胡波[1] 

机构地区：[1]徐州医学院附属医院胸心外科,徐州221002 [2]徐州医学院神经生物学研究中心,徐州221002 [3]徐州医学院附属医院麻醉科,徐州221002

出　　处：《Chinese Medical Journal》2002年第6期878-883,153,共6页中华医学杂志（英文版）

基　　金：agrantfromtheEducationAssociationofJiangsuProvince ,China (No .98JKB32 0 0 0 8)

摘　　要：OBJECTIVE: To investigate the feasibility of endothelialization of bioprosthesis by transfer of vascular endothelial growth factor (VEGF) gene. METHODS: Bovine pericardium treated with glutaraldehyde and L-glutamic acid was positioned into the pig right atrium. pcD(2)/hVEGF(121) gene (1 mg) was transferred into the right ventricular myocardium using surgical sutures Reverse transcri ption polymerase chain reaction (RT PCR) was employed to evaluate the expression of myocardial VEGF mRNA. The determination of concentrations of VEGF protein in blood from both the right atrium and peripheral vein, and histological and ultrastructural analysis of implanted bovine pericardium were completed simultaneously. RESULTS: The concentration of VEGF derived from the right atrium in pcD(2)/hVEGF(121) group was significantly higher than that in the pcD(2) group 10 days after VEGF gene transfer (PObjective To investigate the feasibility of endothelialization of bioprosthesis by transfer of vascular endothelial growth factor (VEGF) gene.Methods Bovine pericardium treated with glutaraldehyde and L-glutamic acid was positioned into the pig right atrium. pcD 2/hVEGF 121 gene (1 mg) was transferred into the right ventricular myocardium using surgical sutures. Reverse transcription-polymerase chain reaction (RT-PCR) was employed to evaluate the expression of myocardial VEGF mRNA. The determination of concentrations of VEGF protein in blood from both the right atrium and peripheral vein, and histological and ultrastructural analysis of implanted bovine pericardium were completed simultaneously.Results The concentration of VEGF derived from the right atrium in pcD 2/hVEGF 121 group was significantly higher than that in the pcD 2 group 10 days after VEGF gene transfer (P<0.01). The expression of myocardial VEGF mRNA in pcD 2/hVEGF 121 group was much higher in comparison with that in the pcD 2 group. The morphological analysis demonstrated that the coverage rate of host endothelium in the pcD 2/hVEGF 121 group was 2.6 times as fast as that in the pcD 2 group at 16 days after VEGF 121 gene transfer (P<0.01). Entire endothelialization occurred at 30 days after VEGF gene transfer. In addition, higher expression of myocardial VEGF mRNA was still available.Conclusions VEGF gene transfer by surgical suture can remarkably accelerate endothelialization of bioprosthesis, which may provide a new approach for inhibiting biological valve calcification and improve biocompatibility and long-term durability of the bioprosthesis.

关 键 词：BIOPROSTHESIS Heart Valve Prosthesis Animals Endothelial Growth Factors Endothelium  Vascular Female Gene Transfer Techniques Humans LYMPHOKINES Male RNA  Messenger Research Support  Non-U.S. Gov't Swine Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors 

分 类 号：R654[医药卫生—外科学] R346[医药卫生—临床医学]