DNA sequence analysis of the triose phosphate isomerase gene from isolates of Giardia lamblia  

蓝氏贾第虫分离株tim基因序列分析比较研究(英文)

作　　者：卢思奇[1] 文建凡[2] 李继红[1] 王凤云[1] 

机构地区：[1]首都医科大学细胞生物学系,北京100054 [2]中国科学院昆明动物研究所细胞与分子进化开放研究实验室,昆明650223

出　　处：《Chinese Medical Journal》2002年第1期99-102,151-152,共4页中华医学杂志（英文版）

摘　　要：Objective To confirm the genetic relation between Giardia lamblia (G. lamblia) isolates from different geographic regions of China and other countries.Methods Genomic DNA were extracted from the trophozoites or cysts of Giardia lamblia. The triose phosphate isomerase (tim) gene was amplified using polymerase chain reaction (PCR) technique. PCR products were digested with endonuclease and sequenced. The data of sequencing were analyzed with the DNAstar software and compared with that of the isolates acquired from GenBank.Results Of nine isolates of Giardia lamblia from China (C1, C2, CH2 and CH3), Cambodia (CAM), Australia (A1 and A2) and America (BP and CDC), respectively, 3 (A1, A2 and CAM) fit into Group 1 (WB), 2 (CH2 and CH3)) into Group 2, and 4 (C1, C2, BP and CDC) into Group 3 (GS). The results confirmed the genetic relatedness of G. lamblia isolates from all over the world.Conclusion Genotyping isolates of G. Lamblia provides important information for establishing the phylogenetic relationship or for the epidemiological evaluation of the spreading of this organism.目的　为了探讨来源于中国和其它国家不同地理位置贾第虫分离株之间的遗传学关系。方法　从贾第虫滋养体和包囊提取总DNA。对磷酸丙糖异构酶基因进行PCR扩增。PCR产物经限制性内切酶消化、序列测定及分析。对所得DNA序列数据用DNAstar软件处理并与登录基因库虫株的序列比较。结果　在来自我国 (C1、C2、CH2、CH3) ,柬埔寨 (CAM)、澳大利亚 (A1、A2 )和美国 (BP、CDC)的 9株蓝氏贾第虫中 ,A1、A2和CAM属于第 1型 (WB) ;CH2和CH3属于第 2型 (JH) ;C1、C2、BP和CDC属于第 3型 (GS)。结论　贾第虫分离株基因型的确定可为本虫分子系统进化和分子流行病学研究提供重要资料。

关 键 词：Giardia lamblia   ·  triose phosphate isomerase   · tim gene   ·  gene sequence analysis   ·   genotype 

分 类 号：R382.2[医药卫生—医学寄生虫学]