Effects of shenmai injection on expression of TNF-α mRNA in peritoneal macrophages of scald mice  

参麦注射液对烫伤小鼠腹腔巨噬细胞TNF-α mRNA表达的影响(英文)

作　　者：王润田[1] 高潮[1] 刘殿武[1] 

机构地区：[1]河北医科大学基础所免疫室,石家庄050017

出　　处：《Chinese Medical Journal》2002年第2期293-295,共3页中华医学杂志（英文版）

基　　金：theEducationDepartmentofHebeiProvince (No .2 0 0 0 112 )

摘　　要：Objective To explore the effect of shenmai injection (SI) on expression of TNF-α mRNA in peritoneal macrophages (pMΦs) of scald mice.Methods BALB/c mice were inflicted with 11% of body surface area Ⅲ degree scald and injected intraperitoneally (ip) with SI daily for 5 days, and expression of TNF-α mRNA in pMΦs was determined by semi-quantitative RT-PCR.Results In scald mice, the expression of TNF-α mRNA in pMΦs increased significantly, but it was reduced obviously (P<0.01) after SI administration, while the livability was increased markedly (P<0.05).Conclusions For scald mice, the cause of death at early stage might be related to the high expression of TNF-α mRNA in pMΦs and the use of SI can decrease the death rate.目的　探讨参麦注射液 (SI)对烫伤小鼠腹腔巨噬细胞TNF αmRNA表达的影响。方法　致BALB/C小鼠占体表面积 11%的Ⅲ度烫伤 ,每日腹腔注射SI,连续 5天。半定量RT PCR测定腹腔巨噬细胞TNF αmRNA表达。结果　烫伤小鼠腹腔巨噬细胞TNF αmRNA表达显著增高 ,而SI可使之显著降低 (P <0 0 1)并明显提高烫伤小鼠的存活率 (P <0 0 5 )。结论　烫伤小鼠早期死亡原因可能与腹腔巨噬细胞TNF αmRNA高表达有关。SI可降低烫伤小鼠腹腔巨噬细胞TNF αmRNA的高表达 ,提高烫伤小鼠存活率。

关 键 词：scald    ·  peritoneal macrophages   ·   tumor necrosis factor-α   ·   shenmai injection 

分 类 号：R285[医药卫生—中药学]