Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China  

南澎列岛恙虫病东方体分离株的PCR/RFLP分析(英文)

作　　者：彭桂福[1] 王志斌[1] 王珊珊[1] 黄佳亮[1] 姜普林[1] 曾年华[1] 刘金华[1] 朱少凡[1] 

机构地区：[1]广州军区军事医学研究所流行病研究室,广州510507

出　　处：《Chinese Medical Journal》2002年第12期1881-1882,155,共2页中华医学杂志（英文版）

摘　　要：OBJECTIVE: To identify genotype of eight strains of Orientia tsutsugamushi (O. tsutsugamushi) isolated from Nan Peng Lie Islands in China and establish tsutsugamushi disease nature foci for this region. METHODS: The nested polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used. Three primers were selected from the DNA sequence of the gene encoding type-specific 56-kDa protein of the Karp strain. The positive products were digested by Hine II and Pst I, meanwhile profiles specific to each strain were analyzed. RESULTS: Three genotypes of O. tsutsugamushi including Karp, Kato and a new strain existed on Nan Peng Lie Islands. CONCLUSION: Nan Peng Lie Islands is tsutsugamushi disease nature foci.目的　为确定我国南澎列岛是否为恙虫病疫源地 ,对来自该地的 8个恙虫病东方体分离株进行基因鉴定。方法　应用套式聚合酶链反应 (NPCR)和限制片段长度多态性分析 (RFLP) ,NPCR的引物来自恙虫病东方体5 6kDa蛋白基因 ,NPCR阳性产物由HincII和PstI消化并产生特异性酶切图谱并予以分析。结果　南澎列岛的 8个恙虫病东方体分离株属三个型 ,即Karp ,Kato和一新型恙虫病东方体。

关 键 词：ANIMALS China DNA  Bacterial GENOTYPE Mice Orientia tsutsugamushi Polymorphism  Restriction Fragment Length Rats 

分 类 号：R513.2[医药卫生—内科学]