香蕉枯萎病菌4个生理小种生化特征的比较  

Comparison of biochemical characteristics among four physiological races of Fusarium oxysporum f.sp.cubense

作　　者：舒灿伟[1] 张源明[1] 曾蕊[1] 周而勋[1] 

机构地区：[1]广东省微生物信号与作物病害防控重点实验室/华南农业大学农学院,广州510642

出　　处：《华中农业大学学报》2017年第3期32-37,共6页Journal of Huazhong Agricultural University

基　　金：国家现代农业(香蕉)产业技术体系建设专项(CARS-32)

摘　　要：为研究香蕉枯萎病菌(Fusarium oxysporumf.sp.cubense)生理小种之间的差异,对该菌的细胞壁降解酶、酯酶同工酶和可溶性蛋白进行测定和分析,结果表明:在4个生理小种中均能检测到4种细胞壁降解酶(PG、PMG、Cx和FPA)。其中4号生理小种的PMG和Cx酶活性最高,且国外的1号和4号生理小种菌株的Cx酶活性比国内同种生理小种菌株的高;国内1号生理小种的FPA酶活性最低,其他小种的酶活性差异不显著;同一生理小种国内外菌株的PG酶活性差异不显著;4个生理小种的酯酶同工酶和可溶性蛋白电泳图谱差异较大,聚类分析表明致病力相近的菌株聚在同一分枝上,亲缘关系较近。To identify the differences among physiological races of Fusarium oxysporum f.sp.cubense(Foc),the cell wall-degrading enzymes,esterase isozyme and soluble protein of this pathogen were tested and analyzed.The results showed that four kinds of cell wall-degrading enzymes including polygalacturonase(PG),pectin methyl-galacturonase(PMG),celluloses(Cx)and filter paper enzyme(FPA)were detected in all the four physiological races of Focfrom domestic and abroad.The PG and Cx activities of Foc 4were the highest.The Cx activities of Foc 1and Foc 4from abroad were higher than those of the same race from domestic.The FPA activity of Foc 1from domestic was the lowest,while there was no significant difference among other races.The PG activity showed no significant difference in the same race from domestic and abroad.The electrophoretograms of esterase isozyme and soluble protein indicted that great difference existed among all the four races.Cluster analysis further demonstrated that the isolates with similar pathogenicity were clustered into the same branch,which had close genetic relationship.

关 键 词：香蕉枯萎病菌 生理小种 细胞壁降解酶 酯酶同工酶 可溶性蛋白 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]