拟南芥受水杨酸下调的新基因的克隆及功能探究  

Cloning and functional analyses of novel genes down-regulated by salicylic acid in Arabidopsis

作　　者：詹栎 胡贞洁 

机构地区：[1]华中农业大学生命科学技术学院,武汉430070

出　　处：《华中农业大学学报》2017年第5期25-29,共5页Journal of Huazhong Agricultural University

基　　金：中央高校基本科研业务费专项(2662015PY064)

摘　　要：以水杨酸处理后的拟南芥(Col-0)为材料,采用高通量测序技术分析拟南芥在水杨酸处理后的转录组变化,发现了12个受水杨酸下调的新基因。以Col-0幼苗的cDNA为模板克隆其中一个基因NG314,并对其功能进行研究。NG314的cDNA序列总长为1 097bp,基因组DNA包含1个内含子。荧光定量PCR结果表明,NG314的表达量随着水杨酸处理时间延长而逐渐下降,病原菌侵染也能抑制NG314的表达。NG314可能是一个长链非编码RNA或新的miRNA基因,可能通过抑制其靶基因的表达调控植物的抗病反应。High-throughput sequencing was used to study the transcriptomic changes of wild-type arabidopsis(Col-0)after salicylic acid treatment.12 novel genes down-regulated by salicylic acid were identified.Among them,NG314 was studied and cloned from the cDNA of Col-0seedlings.The total length of NG314 cDNA sequence is 1 097 bp.The genomic DNA of NG314 contains one intron.Results of fluorescence quantitative PCR analyses showed that the expression of NG314 gradually decreased under the salicylic acid treatment.Pathogen infection repressed the expression of NG314.It is indicated that NG314 may encode a long non-coding RNA or novel miRNA,and may regulate plant disease resistance through repressing the expression of its target genes.

关 键 词：拟南芥 水杨酸 NG314基因 病原菌 非编码RNA MIRNA 

分 类 号：Q943.2[生物学—植物学] S432.23[农业科学—植物病理学]