聚合酶链反应-限制性片段长度多态性检测肿瘤坏死因子β基因多态性  

Detection of Tumor Necrosis Factor-beta Gene Polymorphism by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism

作　　者：鞠少卿[1] 王惠民[1] 钱绩虎[2] 

机构地区：[1]南通医学院附院检验科,江苏南通226001 [2]南通医学院免疫学教研组,江苏南通226001

出　　处：《现代检验医学杂志》2002年第4期20-21,共2页Journal of Modern Laboratory Medicine

摘　　要：目的 建立聚合酶链反应限制性片段长度多态性(PCR—RFLP)检测肿瘤坏死因β(TNFβ)多态性的方法。方法 采用PCR方法扩增TNFβ第一外显子到第二外显子包含G→A突变在内的DNA片段,扩增产物用限制性内切酶Nco I酶切后电泳,分析TNFβ的基因型。结果 TNFβ检测到3种基因型,分别为TNFβ*1/1、TNFβ*1/2和TNFβ*2/2。结论PCR—RFLP检测肿瘤坏死因子β多态性方法快速、简便、准确、可靠,适合普通实验室开展及大规模研究。Objectives To establish polymerase chain reaction-restriction fragment length polymorphism to analyze the polymorphism of tumor necrosis factor-beta (TNF β)gene. Methods Polymerase chain reaction (PCR) was performed to amplify spanning exon 1 to exon 2 of TNFβ gene including G→A mutation. PCR production was digested with restriction endonuclease after electrophorsis TNF β genotype were analyzed. Results Three kinds of genotypes at TNF β locus detected were TNF β*1/1, TNF β* 1/2 and TNF β* 2/2. Conclusion PCR-RFLP is a rapid and simple method for obtaining accurate and raliable result of TNF β. It is suitable for the routine and extensive study work in ordinary laboratory.

关 键 词：肿瘤坏死因子Β 基因多态性 聚合酶链反应 限制性片段长度 

分 类 号：R446.61[医药卫生—诊断学] Q346[医药卫生—临床医学]