药物干预对杀虫双染毒大鼠全血胆碱酯酶活力的影响  

The activity of blood cholinesterase in rats exposed to dimehypo after drug intervention

作　　者：万伟国[1] 徐麦玲[1] 邹和建[1] 陆爱玲[1] 沈新玉[1] 陈宇明[1] 

机构地区：[1]复旦大学附属华山医院职业病科,上海200040

出　　处：《中华劳动卫生职业病杂志》2002年第6期419-421,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的　研究氯解磷定和二巯丙磺钠对杀虫双中毒大鼠胆碱酯酶 (ChE)活力的影响。方法　对照组及干预组的Wistar大鼠按杀虫双染毒剂量各分为 4组 (共 1 2组 ) ,在杀虫双经口染毒前及染毒后 1 / 2、1、2、4、2 4h取大鼠尾尖血分别测定ChE活力。干预组在染毒后即刻于大鼠臀部肌内注射氯解磷定 (剂量为 40mg/kg)或二巯丙磺钠 (剂量为 4mg/kg) ,同样采血测ChE活力。结果　大鼠经口杀虫双染毒后 1h ,对照组 4个染毒剂量组的ChE活力分别为 (1 .0 4± 0 .2 1 )、(0 .84± 0 .1 2 )、(0 .71±0 .1 2 )、(0 .66± 0 .0 7)U/ml;氯解磷定干预组为 (1 .0 1± 0 .1 8)、(1 .1 7± 0 .1 1 )、(1 .0 1± 0 .0 4 )、(1 .0 3±0 .1 2 )U/ml;二巯丙磺钠干预组为 (1 .1 5± 0 .1 5)、(1 .2 6± 0 .2 7)、(1 .0 8± 0 .0 8)、(1 .0 4± 0 .1 2 )U/ml。氯解磷定干预组和二巯丙磺钠干预组都可使受抑制的ChE活力部分恢复 ,两组间差异无显著性 (P >0 .0 5) ,在高剂量染毒组 (1 / 4LD50 与 1 / 2LD50 )二者的复能作用均稍差。Objective To investigate the activity of ChE in rats poisoned by dimehypo and then treated with pralidoxime methylchloride or unithiol. Method Rats were divided into control group(dimehypo);intervention groups[dimehypo plus pralidoxime methylchloride or dimehypo plus unithiol(sodium dimercaptopropanesulphonate)].Rats were dosed with 4 different doses of dimehypo:1/16,1/8,1/4 and 1/2 of LD 50 respectively(the LD 50 of dimehypo is 342 mg/kg).After being poisoned with dimehypo orally,rats were immediately injected intramuscularly with pralidoxime methylchloride or unithiol.The activity of ChE in blood was detected before and 1/2,1,2,4 and 24 h after poisoning in dimehypo and intervention groups. Result The ChE cctivity of four dose subgroups at 1 h after poisoning were (1.04±0.21),(0.84±0.12),(0.71±0.12),(0.66± 0.07) U/ml respectively;the ChE activity of pralidoxime methylchloride intervention groups were (1.01±0.18),(1.17± 0.11) ,(1.01±0.04),(1.03±0.12)U/ml respectively;and the ChE activity of unithiol intervention groups were (1.15±0.15),(1.26±0.27),(1.08±0.08),(1.04±0.12)U/ml respectively.The inhibited ChE in blood was recovered by either treatment with pyraldoxime methylchloride or unithiol.These two drugs had similar effects of recovering the activity of ChE( P > 0.05) ,but at higher doses(1/4 and 1/2 of LD 50 ) the effects of both were not so good. Conclusion Pralidoxime methylchloride and unithiol could partly recover the activity of ChE inhibited by dimehypo .

关 键 词：药物干预 大鼠 杀虫药 沙蚕毒 胆碱酯酶类 解磷定化合物 二巯丙磺钠 农药中药 

分 类 号：R595.4[医药卫生—内科学]