高原鼢鼠肌肉脂溶性物质对大鼠一氧化氮合成酶活性的影响  

The effects of fat soluble component extracted from the muscle of Plateau Zokor(Myospalax baileyi) on the activities of nitric oxide synthase in rat

作　　者：魏登邦[1] 张宝琛[1] 

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810001

出　　处：《青海大学学报（自然科学版）》2002年第6期33-35,共3页Journal of Qinghai University(Natural Science)

基　　金：中国科学院院长基金特别资助项目

摘　　要：为了探讨高原鼢鼠肌肉脂溶性物质 (FSC)的抗缺氧机制 ,40只SD大鼠随机分为 4组 :1 0 %FSC组、1 0 %FSC缺氧组、空白对照组和空白缺氧组。FSC组和空白组分别用 1 0 %FSC和蒸馏水连续灌胃 3 0d ,剂量为每天 2 0mL kg。灌胃结束后 ,1 0 %FSC缺氧组和空白缺氧组大鼠在 5 0 0 0m海拔高度下 ,连续缺氧 1 5d ,每天 8h ,测定各组大鼠血清一氧化氮合成酶 (NOS)活性。结果发现 ,在 5 0 0 0m海拔高度条件下 ,1 0 %FSC在缺氧条件下能显著提高大鼠血清NOS活性和NO含量 (P <0 0 5 )。The fat soluble component (FSC) extracted by means of supercritical fluid extraction from plateau zokor muscle had significant anti-hypoxic function.To explore anti-hypoxic mechanisms of FSC,forty SD rats were divided randomly into four groups,including FSC experimental groups (10% FSC group,10% FSC+hypoxia group) and megative control groups (control group,control+ hypoxia group),the FSC expermental groups and negative control groups were fed with 10% FSC and distilled water respectively,each animal receiving a constant daily does of 20 mL/kg body weight for thirty days consecutively.After finishing the treatment,the hypoxic groups were exposed to hypoxic condition(at the altitude of five thousand meters) eight hours daily for fifteen consecutive days.The activities of nitric oxide synthase (NOS) and the contents of nitric oxide (NO) in serum were determined.The results showed that the activities of NOS and the contents of NO increased markedly in serum of 10% hypoxic FSC group compared with that of hypoxic control group at the altitude of five thousand meters.

关 键 词：大鼠 酶活性 高原鼢鼠 脂溶性物质 一氧化氮合成酶 抗缺氧机制 生物医学 

分 类 号：Q559[生物学—生物化学]