根据SSR标记划分优质蛋白玉米自交系的杂种优势群  

Heterotic Grouping of Quality Protein Maize Inbreds Divided by SSR Markers

作　　者：番兴明[1] 张世煌[2] 谭静[1] 李明顺[2] 李新海[2] 

机构地区：[1]云南省农业科学院粮食作物研究所,云南昆明650205 [2]中国农业科学院作物育种栽培研究所,北京100081

出　　处：《作物学报》2003年第1期105-110,共6页Acta Agronomica Sinica

基　　金：云南省自然科学基金资助项目 (98C0 0 6Z)

摘　　要：利用 SSR标记技术对 18个优质蛋白玉米 (QPM)自交系和 4个代表国内主要杂种优势群的普通玉米标准测验种进行杂种优势群划分 ,研究热带、亚热带 QPM与温带玉米自交系之间的遗传关系。从 70对引物中筛选出 39对扩增谱带清晰且具有多态性的 SSR引物 ,在供试材料中检测到 134个等位基因变异 ,平均多态性信息量为 0 .5 5。根据扩增谱带建立 0、 1型数据 ,计算 2 2个自交系间的遗传相似值 ,然后做聚类分析。结果表明 ,供试的 18个 QPM自交系可划分为 5群 :第一群与旅大红骨种质的遗传距离较近 ,包括 CML 14 9、 CA339、 CML 15 4、长 6 31/o2、中系 0 96 /o2、CML 16 6和 CML 16 4。第二群接近四平头种质 ,包括 CML 14 0、 YML 2 3、 YML 2 9和 CML 194。第三群接近瑞得种质 ,包括忻 910 1/o2和齐 2 0 5。第四群与兰卡斯特种质的距离较近 ,包括 YML 12和 YML 10 2。第五群与四个主要杂种优势类群的距离都比较远 ,包括 CML 14 7、 CML 16 1和 CML 171。 SSR标记划群与田间产量配合力划分结果及系谱分析基本一致。Heterotic grouping of 22 maize inbred lines, including 18 quality protein maize (QPM) lines and 4 domestic temperate common testers that represent four heterotic groups used dominantly in China, were classified by SSR markers. A set of 39 polymorphic SSR primers were selected from 70 primers, which detected 134 alleles among the lines. Mean polymorphism information content was 0.55. Cluster analysis was based on the genetic similarity coefficient. Five heterotic groups were determined among the 22 inbred lines: group 1 was of Luda Red cob germplasm including CML149, CA339, CML154, Chang 631/o2, Zhongxi096/o2, CML166 and CML164. Group 2 was of Sipingtou germplasm including CML140, YML23, YML29 and CML194. Group 3 was of Reid germplasm including Xin 9101/o2 and Qi 205. YML12 and YML102 were included in Lancaster group. CML147, CML161 and CML171 can not be included in the current groups. The results basically were agreeable with the previous studies based on combining ability analysis for yield and available pedigree data.

关 键 词：SSR标记 蛋白玉米 自交系 杂种优势 遗传相似系数 

分 类 号：S513[农业科学—作物学]