大豆异黄酮抑制裸鼠移植瘤生长及其血管生成的实验研究  

Experimetal study on growth inhibition and angiogenesis in transplanted tumor caused by genistein in vivo

作　　者：河福金[1] 王健[1] 牛建昭[1] 白锦雯[1] 杨美娟[1] 赵丽云[1] 王继峰[1] 

机构地区：[1]北京中医药大学细胞与生化实验室,北京100029

出　　处：《中国药理学通报》2003年第1期73-76,共4页Chinese Pharmacological Bulletin

基　　金：国家教育部博士学科点专项科研基金资助 No 2 0 0 0 0 2 60 4

摘　　要：目的　探讨大豆异黄酮及其主要有效成分金雀黄素对人乳腺癌细胞裸鼠移植瘤的生长及其血管生成的影响。方法　选用人乳腺癌细胞株MCF 7裸鼠异种移植 ,观测移植瘤的重量、出瘤时间、成瘤率 ,同时应用FⅧ免疫组化染色 ,观测瘤组织内的新生血管。结果　FⅧ免疫组化染色结果表明 ,肿瘤组织内可见形态不规则 ,且无明显管腔的新生血管 ,血管分布以肿瘤组织的边缘处为多见。各组微血管记数结果金雀黄素组最少。肿瘤出瘤时间及成瘤率各防治组都明显延长和减少 ,以金雀黄素组最为明显 ,其次是高黄酮组和低黄酮组。结论　大豆异黄酮的主要有效成分金雀黄素能减少肿瘤的血管生成 ,从而抑制裸鼠移植瘤的体内生长。AIM To study growth inhibition and expression level of VEGF in tumor tissue transplanted with human breast cancer cell line by genistein in vivo . METHODS Human breast cancer cell line (MCF 7) was transplanted into nude mice. Immunochemistry staining for factor Ⅷ was performed. The weight of tumor, latent period and percent of tumor development were observed. RESULTS Immunochemistry staining showed irregular angiogenesis without obvious tynicavity mostly scattered around edge of tumor. The count of microvessel was the fewest in genistein group. For genistein treatmented group, latent period was the longest and percent of tumor development was the lowest. CONCLUSION Genistein inhibits angiogenesis in breast cancer transplanted in mice, so that growth of transplanted tumor can be inhibited in vivo .

关 键 词：大豆异黄酮 金雀黄素 MCF-7细胞 血管生成 

分 类 号：R285[医药卫生—中药学]