中缝背核投射到基底外侧杏仁核的5-羟色胺能纤维对睡眠的调节作用  

Role of serotonergic projection from dorsal raphe nuclei to basolateral amygadaloid in regulation of sleep

作　　者：赵乐章[1] 高隽[1] 张景行[1] 章功良[1] 钟明奎[1] 张谨[1] 

机构地区：[1]安徽医科大学生理学教研室,合肥230032

出　　处：《中国药理学通报》2003年第1期77-79,共3页Chinese Pharmacological Bulletin

基　　金：安徽省教委自然科研基金 (No 2 0 0 0J10 96);安徽医科大学校基金(No 2 0 0 10 0 5 )资助项目

摘　　要：目的　观察大鼠中缝背核 (DRN)到基底外侧杏仁核(BLA)的 5 HT能纤维投射在睡眠 -觉醒调节中的作用。方法　采用脑立体定位 ,核团微量注射和多导睡眠描记 (PSG)方法。结果　DRN内微量注射L Glu ,可使觉醒 (W )增加 ,慢波睡眠 (SWS)和异相睡眠 (PS)明显减少。在双侧BLA微量注射非选择性 5 HT受体阻断剂麦角新碱 (MS)可以逆转DRN内微量注射L Glu的效应 ,SWS增加 ,W减少 ,但PS没有变化 ;DRN内微量注射PCPA ,导致SWS增加 ,W减少 ,但当在DRN内微量注射PCPA后 ,双侧BLA内微量注射 5 HTP可以逆转PCPA所引起的睡眠增加效应 ,使SWS减少 ,W增加 ,但PS没有变化。结论　DRN对睡眠 -觉醒的调节作用部分通过DRN到BLA的 5AIM To investigate the role of serotonergic projection from dorsal raphe nuclei (DRN) to basolateral amygadaloid (BLA) in the regulation of sleep and waking state. METHODS stereotaxic microinjection and polysomnography were employed. RESULTS Microinjection of L Glu into the DRN caused an enhancement of wake (W) and a decrease of slow wave sleep (SWS) and paradoxical sleep (PS). However, microinjection of L Glu into the DRN plus bilateral microinjection of methysergide (MS) into the BLA reversed the effects of L Glu. Microinjection of PCPA into the DRN caused an enhancement of SWS and a decrease of W. Microinjection of PCPA into the DRN plus bilateral microinjection of 5 HTP into the BLA reversed the effects of PCPA. CONCLUSION These results suggest that the role of the DRN in the regulation of sleep and waking state is partly mediated by serotonergic projection from the DRN to the BLA.

关 键 词：中缝背核 基底外侧杏仁核 5-羟色胺 多导睡眠描记 睡眠 

分 类 号：R338.63[医药卫生—人体生理学]