表没食子儿茶素没食子酸酯对肾缺血再灌注损伤的保护作用  

Protective effects of (-)-epigallocatechin-3-gallate on renal ischemia-reperfusion injury

作　　者：梁钢[1] 黄巨恩[2] 张肃[1] 廖铏光 许盈[3] 黄仁彬[1] 

机构地区：[1]广西医科大学药理学教研室,南宁530021 [2]广西医科大学药理学教研室组胚学教研室,南宁530021 [3]广西中医学院药学系,97级本科实习生南宁530001

出　　处：《中国药理学通报》2003年第1期97-100,共4页Chinese Pharmacological Bulletin

基　　金：广西教育厅科研基金资助课题 No桂科研 (2 0 0 1) 40 1号

摘　　要：目的　探讨表没食子儿茶素没食子酸酯 (EGCG)对大鼠肾缺血再灌注损伤的影响。方法　通过结扎肾动脉 60min后再灌注 ,建立大鼠肾缺血再灌注损伤模型 ,给药组于结扎前后分别静脉给予 10mg·kg-1和 40mg·kg-1EGCG。化学法观察大鼠血清肌酐 (Scr)、尿素氮 (Bun)、丙二醛(MDA)含量 ,肾组织内MDA、活性氧 (ROS)含量和超氧化物歧化酶 (SOD)、Ca2 + ATP酶活性的变化 ,并观察肾组织的病理变化。结果　与模型对照组比较 ,40mg·kg-1的EGCG组可抑制由肾缺血再灌注引起的血清Bun、Scr、MDA含量和组织MDA、ROS含量的变化 ,增强SOD和Ca2 + ATP酶活性 ,减轻肾组织病理改变。结论　EGCG有保护大鼠肾缺血再灌注损伤的作用 。AIM To study the protective effects of ( ) epigallocatechin 3 gallate (EGCG) against renal ischmia reperfusion injury. METHODS The renal ischemia reperfusion injury model in rat was made by means of ligating bilateral renal arteries for 60 min and then reperfusion. 10 mg·kg -1 and 40 mg·kg -1 EGCG were given by intravenation before and after operation respectively. Spectrophotometric assay were used to measure the contents of serum creatinine(Scr), blood urea nitrogen (Bun) in serum and the activity of superoxide dismutase (SOD) and Ca 2+ ATPase ,the contents of malondialdehyde (MDA), reactive oxygen species (ROS) in renal hemogenate from rats. Renal pathologic changes were also observed. RESULTS Compared with model control group , 40 mg·kg -1 EGCG group was found of significant inhibition in changes of Scr, Bun,MDA,ROS and significant increases in activity of SOD and Ca 2+ ATPase.The renal morphological injury of EGCG groups were slighter than that of model control group. CONCLUSION EGCG has protective effects aganist renal ischemia reperefusion injury via its antioxidant activity and intracellular caclcium reduction.

关 键 词：表没食子儿茶纱没食子酸酯 肾缺血再灌注损伤 抗氧化 

分 类 号：R692.5[医药卫生—泌尿科学] R965[医药卫生—外科学]