肺炎支原体肺炎及支气管哮喘HLA-DRB1基因位点频率研究  

Study on gene frequency of hla-drb1expression in children with mycoplasma pnEumonia and bronchial asthma

作　　者：李琳[1] 曹兰芳[1] 顾梯成[1] 陆伟蓉[1] 

机构地区：[1]上海第二医科大学附属仁济医院儿科,上海200001

出　　处：《临床儿科杂志》2003年第1期29-31,共3页Journal of Clinical Pediatrics

基　　金：上海市科技发展基金资助 (项目编号 :004119050)

摘　　要：为探讨肺炎支原体 (MP)肺炎的免疫致病机理及其与支气管哮喘 (简称哮喘 )之间的关系 ,应用颗粒凝集法检测29例MP肺炎患儿、40例哮喘患儿及92例正常对照组的血清MP特异性IgM抗体 ,PCR_SSOP法标记HLA_DRB1等位基因位点。结果显示MP肺炎组、哮喘组的DRB1 08基因频率明显低于正常对照组 (P=0 .035) ,但MP肺炎组与哮喘组之间差异无显著性 ,哮喘组中除DRB 08外 ,DRB1 02基因出现频率亦较正常人显著降低 (P=0 .041) ;各组间HLA_DRB1 04、07、09、10、11、12基因频率虽有所变化 ,但均无统计学差异。to investigate the immuno_pathogenesis of mycoplasma pneumoniae(Mp)pneumonia and its association with bronchial asthma(asthma),serum mp_specifIc igm antibody was detected by means of particle agglutination test and also hlA_drb1alleles was amplified by means of pcr_ssop assay in children with mp pneumOnia(29cases),with asthma(40cases)and healthy people(92cases)as normal coNtrols.the results indicated that gene frequency of hla_drbl ˇ 08exˉpression oF children with both mp pneumonia(6.90%)and asthma(1.25%)was evidently lower than that of normal controls(10.33%,p=0.035).but,there was no significant dIfference betwwen these2affected groups.meanwhile,the gene freˉquency of hla_dRb1 ˇ 02expression of children with asthma was significantly lower than that in normal controls(p=0.041).in addition,no statistical difference could be discOvered in other different hla_drb1 ˇ 04,07,09,10,11and12 among these3groups,although some alteration in the gene frequency of these ALlele expression actually apˉpeared.therefore,it might be suggested that mp pnEumonia has a same immunopathogenetic background just as that apˉpearing in chiLdren with asthma.

关 键 词：肺炎支原体肺炎 支气管哮喘 人类白细胞抗原-DRB1 儿童 颗粒凝集法 

分 类 号：R725.6[医药卫生—儿科]