抗CD3、CD7单抗-PAPs免疫毒素的构建及其联合杀伤效应  

Construction and Combined Cytotoxicity of Anti-CD3 and Anti-CD7 Monoclonal Antiboby-PAPs Immunotoxin

作　　者：张玲[1] 李官成[2] 朱兆玲[2] 林学颜[2] 

机构地区：[1]中山大学肿瘤防治中心肿瘤研究所病因研究室,广东广州510060 [2]中山大学免疫学教研室,广东广州510080

出　　处：《中山医科大学学报》2003年第1期39-41,共3页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：广东省科委重点攻关课题资助项目(97-77-5)

摘　　要：[目的]应用抗人T淋巴细胞单抗(抗CD3和抗CD7),分别与商陆抗病毒蛋白(PAPs)偶联构成两种免疫毒素,并探讨这两种免疫毒素在体外联合对T淋巴细胞和T淋巴母细胞(CEM细胞)的杀伤效应。[方法]选用抗CD3和抗CD7杂交瘤细胞制备单克隆抗体腹水,经纯化后以SPDP为交联剂,将两种单抗分别与商陆抗病毒蛋白偶联构建成抗CD3和抗CD7单抗免疫毒素;分别以及联合后对T淋巴细胞和CEM细胞进行体外细胞毒试验。[结果]构建的两种单抗-PAPs免疫毒素活性良好,其特异性杀伤T淋巴细胞和T淋巴母细胞CEM细胞的效应比单一抗CD3-PAPs和抗CD7- PAPs更强,对照组仅有轻微的杀伤效应。[结论]成功地构建了抗 CD3和抗 CD7单抗-PAPs免疫毒素,及其不同抗原决定簇的单抗免疫结合物,并证明联合应用抗CD3-PAPs和抗CD7-PAPs免疫毒素有相加的杀伤效果。[Objective] To construct two monoclonal antibody immunotoxins of anti-CD3-PAPs and anti-CD7-PAPs, and to test its cytotoxicity on T lymphocyte and T lymphoblast (CEM cell). [Methods] Purified anti-CD3 and anti-CD7 monoclonal antibodies (McAb) were linked with PAPs by SPDP, and constructed immunotoxin of anti-CD3-PAPs and anti-CD7-PAPs. The cytotoxicity of two immunotoxins on CEM cells was tested respectively and jointly. [Results] The bi-McAb-immunotoxin showed higher activities and specific killing effect on CEM cell and T lymphocytes than anti-CD3-PAPs or anti-CD7-PAPs alone. Lower cytotoxicity was found in control group. [Conclusion] Anti-CD3-PAPs and anti-CD7-PAPs immunotoxin were constructed and showed higher cytotoxicity on T lymphocytes when they were used jointly than used alone.

关 键 词：免疫毒素类 商陆有丝分裂原类 单克隆抗体 T淋巴细胞 杀伤效应 

分 类 号：R392.11[医药卫生—免疫学]