病毒膜蛋白及其表达和纯化技术的研究进展  

ADVANCES IN VIRAL ENVOLOPE STUDY AND ITS EXPRESSION AND PURIFICATION

作　　者：解飞霞[1] 宋晓玲[2] 黄倢 徐怀恕[1] 

机构地区：[1]青岛海洋大学,266003 [2]中国水产研究院黄海水产研究所,青岛266071

出　　处：《海洋湖沼通报》2002年第4期76-82,共7页Transactions of Oceanology and Limnology

摘　　要：病毒囊膜是包膜病毒除核衣壳外的另一主要成分。它主要由多种蛋白质构成,称为膜蛋白。膜蛋白以单聚体、二聚体、三聚体,甚至更多的聚合体的形式存在,具有中和抗体、与靶细胞受体结合、凝集红细胞、诱导细胞融合等多种功能。膜蛋白可采用将其基因整合入载体,并在昆虫细胞、哺乳细胞或细菌中获得体外表达。膜蛋白可在去污剂的作用下形成蛋白质-脂-去污剂的复合物而被溶解,其中去污剂的临界微团浓度、亲水物-亲脂物平衡值、荷电、紫外穿透性等是选择合适去污剂的主要参考因子。溶解后的膜蛋白多利用聚丙烯酰胺凝胶电泳、凝胶过滤层析、离子交换层析、亲和层析、高效液相层析等技术并结合梯度离心、分级沉淀等进行分离、纯化。Viral envelope is an important component of enveloped viruses. Envelope protein exists as monomer, dimer, trimer,even more complicate polymer. Envelope protein has many functions, such as neutralizing antibody, binding target cells, hemagglutinating red blood cells and inducing cells fusion etc. After ligating to a vector, envelope protein genes can be expressed in insect cells,mammalian cells and bacteria. Treated with detergent, envelope protein can be solubilized as protein-lipid-detergent complex. CMC, HLB, cloud point and UV-transparency are influential regents of detergent in solubling envelope protein. Solubilized envelope protein can be extracted and purified through gradient centrifugation, grade-sedimentation together with SDS-PAGE, gel-filtration, ion-exchange, affinity chromatography and HPLC.

关 键 词：纯化技术 病毒膜蛋白 结构 功能 表达 分离 

分 类 号：Q503[生物学—生物化学] Q78