异氟醚对人肺泡巨噬细胞趋化因子和细胞因子mRNA表达的影响  

Expression of Genes for Cytokines and Chemokine in Alveolar Macrophages during Isoflurane Anesthesia

作　　者：张光明[1] 蒋豪[1] 薛张纲[1] 方浩[1] 王文逸[2] 瞿涤[2] 

机构地区：[1]复旦大学附属中山医院麻醉科,上海,200032 [2]复旦大学附属中山医院教育部医学分子病毒学重点实验室,上海,200032

出　　处：《复旦学报（医学版）》2003年第1期4-8,共5页Fudan University Journal of Medical Sciences

基　　金：上海市领先科学基金资助项目 ( 9930 2 7)

摘　　要：目的　研究异氟醚麻醉中人肺泡巨噬细胞IL 8、IL 1β、TNF α、IFN γ、IL 4和IL 10mRNA的表达 ,探讨异氟醚对肺炎性反应的影响。方法　 2 4例肝脏部分切除手术病人随机分为异氟醚组 (n =12 )和对照组 (n =12 ) ,在麻醉诱导后即刻和 4h分别用纤维支气管镜行支气管肺泡灌洗 ,从灌洗液中收获肺泡巨噬细胞提取RNA ,逆转录合成cDNA以 β actin为内对照作PCR后电泳扫描求积 ,检测肺泡巨噬细胞上述细胞因子、趋化因子mRNA的表达。结果　异氟醚组麻醉诱导后 4h比麻醉诱导后即刻IL 8、IL 1βmRNA的表达显著增加 (P <0 .0 5 ) ,对照组麻醉诱导后 4h比麻醉诱导后即刻IL 8、IL 1βmRNA的表达轻度增加 (P <0 .0 5 ) ;在麻醉诱导后4h ,异氟醚组和对照组之间IL 8、IL 1βmRNA的表达水平差异有显著意义 (P <0 .0 5 )。TNF α、IFN γ、IL 4和IL 10mRNA的表达在麻醉前后无明显变化。结论　异氟醚能增强病人肺泡巨噬细胞促炎性细胞因子、趋化因子mRNA的表达 。Purpose: To investigate whether isoflurane cause lung inflammation through observing the changes of interlukin-8(IL-8), IL-1β, tumor necrosis factor-α(TNF-α), interferon-γ(IFN-γ), IL-4 and IL-10 mRNA expressions in alveolar macrophages during isoflurane anesthesia. Methods: Patients undergoing hepatectomy were anesthetized with isoflurane(n = 12) or control(n = 12) combined anesthesia. Alveolar macrophages were harvested by bronchoalveolar lavage immediately and 4 h after induction of anesthesia. RNA was extracted from harvested cells and cDNA was synthesized by reverse transcription. Expressions of IL-8, IL-1β, TNF-α, IFN-γ, IL-4 and IL-10 mRNA was measured by semi-quantitative polymerase chain reaction using β-actin as an internal standard. Results: Gene expression of IL-8 and IL-1β in alveolar macrophages increased significantly at 4 h after induction of anesthesia in isoflurane group(P<0.05). The increase was slight in control group. The differences of IL-8, IL-1β gene expressions between isoflurane and control groups are significant. There were no changes among the expressions of TNF-α, IFN-γ, IL-4 and IL-10 mRNA for both groups during anesthesia. Conclusions: Isoflurane can increase the expression of genes for cytokines and chemokine such as IL-8 and IL-1β in alveolar macrophages. Inflammatory responses in lung can be augmented by isoflurane.

关 键 词：肺泡巨噬细胞 趋化因子 异氟醚 细胞因子 逆转录-聚合酶链反应 支气管肺泡灌洗 麻醉 RT-PCR 

分 类 号：R971.2[医药卫生—药品] R614.2[医药卫生—药学]