重组幽门螺杆菌尿素酶B亚单位疫苗鼻腔免疫的实验研究  

Immunopotency of the recombinant urease B subunit vaccine of Helicobacter pylori after intranasal administration to mice

作　　者：高志刚[1] 邹全明[1] 郭刚[1] 郭桐生[1] 曾韦锟[1] 解庆华[1] 

机构地区：[1]第三军医大学临床微生物及免疫学教研室,重庆400038

出　　处：《细胞与分子免疫学杂志》2003年第1期68-70,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家"九五"重点科技攻关项目(No.969010154);国家"863"计划资助项目(No.2001AA215161)

摘　　要：目的:探讨基因工程疫苗Hp重组尿素酶B亚单位(rUreB)鼻腔接种的免疫效果。方法:以rUreB不同剂量或加不同佐剂滴鼻免疫BALB/c小鼠。末次免疫7 d后,收集血清及胃黏膜、小肠黏膜、鼻黏膜及气管黏膜冲洗液,用ELISA法检测抗rUreB特异性抗体。结果:rUreB鼻腔免疫后各实验组血清特异性IgG及各黏膜冲洗液中特异性IgA的水平均明显增高,与对照组相比较差异显著(P<0.01)。20μg剂量组与10μg剂量组相比较,仅血清特异性IgG水平增高,其它黏膜特异性IgA的水平未见增高。大肠杆菌不耐热肠毒素B亚单位(LTB)的佐剂效果较霍乱毒素B亚单位(CTB)强,卡泊波可增强鼻腔接种疫苗在胃黏膜洗液中的抗体应答水平。结论:CTB、LTB、卡泊波均可作为rUreB鼻腔黏膜接种的佐剂。HprUreB鼻黏膜接种,不仅可诱导血清特异性抗体反应,而且能引起多个黏膜部位的免疫应答,是一种方便、有效、廉价的免疫途径。AIM: To observe the immunopotency of the recombinant urease B subunit ( rUreB) of Helicobacter pylori after intranasal administration to mice. MDTHODS: BALB/c mice were immunized intranasally with rUreB of 20 ug, 10 ug and rUreB plus different adjuvants, such as cholera toxin B subunit (CTB) , Escherichia coli heat-labile enterotoxin B subunit (LIB) and carbopol respectively, four times at an intervals of 7 days. The serum and washing solution from gastric, intestinal, nasal and tracheal mucosas were collected in 7 days after final immunization. IgG and IgA antibodies specific for rUreB were detected by ELISA. RESULTS: The levels of IgA and IgG antibodies in sera every groups of mice immunized intranasally were significantly increased compared with control group (P<0.01). Only the levels of serum IgG of mice immunized with 20 ug dose were higher than those of mice immunized with 10 ug dose. Carbopol could enhance the level of IgA antibodies in washing solution from mouse gastric macosa after intranasal immunization . The efficacy of LTB as a nasal mucosal immuno-adjuvant was stronger than that of CTB. CONCLUSION; CTB, LTB and carbopol can play the role of adjuvant in nasal mucosal immunization. Intranasal immunization with rUreB can induce not only serum IgG antibody production but also antibody responses of different mucosa. Thus intranasal inoculation is a convenient, effective and cheap immunization way.

关 键 词：重组幽门螺杆菌尿素酶B亚单位 疫苗 鼻腔免疫 实验研究 

分 类 号：R378[医药卫生—病原生物学]