4种肾炎模型大鼠尿液中补体C5b-9复合物排泄的比较研究  

A comparative study on urinary complement C5b-9 complex excretion in four nephritis models of rats

作　　者：王迎伟[1] 简洁[2] 汤仁仙[2] 黄水平[3] 

机构地区：[1]南京医科大学免疫学教研室 [2]江苏徐州医学院免疫学教研室,江苏徐州221002 [3]江苏徐州医学院统计学教研室,江苏徐州221002

出　　处：《中国病理生理杂志》2003年第1期92-96,I003,共6页Chinese Journal of Pathophysiology

基　　金：江苏省教委基金资助项目 (No .960 95);江苏省自然科学基金资助课题(No.BK971 54);江苏省"333"工程基金资助项目 (No .2 0 0 78)

摘　　要：目的 :探讨不同类型的免疫复合物肾炎大鼠尿液中补体C5b - 9复合物检出的意义。方法 :复制大鼠被动型Heymann肾炎 (PHN)、抗胸腺细胞血清性肾炎 (ATSN)、抗肾小球基底膜性肾炎(AGBMN)及慢性血清病肾炎 (CSDN) 4种动物模型。用双抗体夹心ELISA法定期检测各种肾炎大鼠血液及尿液中补体C5b - 9的含量 ,同时观察大鼠肾小球中C5b - 9的沉积情况。结果 :4种肾炎模型鼠血浆C5b - 9复合物均见上升 ,肾小球内也有C5b - 9复合物的沉积 ,但尿液中C5b - 9含量升高仅见于罹患PHN的大鼠。此外 ,尿液中C5b - 9复合物的升高先于PHN大鼠尿蛋白的升高。结论 :尿液中C5b -AIM: To explore the significance of measuring urinary complement C5b-9 complex in various types of immune complex (IC) nephritis models of rats. METHODS: The four models of rats, namely, passive Heymann nephritis (PHN), anti-thymocyte serum nephritis(ATSN), anti-glomerular basement membrane nephritis (AGBMN) and chronic serum disease nephritis (CSDN) were reproduced. Then, the contents of complement C5b-9 complex in plasma and urine of the rats were detected with sandwich ELISA. And the deposits of C5b-9 complex in glomeruli of the rats were examined by ABC immunohistochemistry staining. RESULTS: The contents of rat plasma C5b-9 were elevated and deposits of C5b-9 in glomeruli could be detected in the four model rats. But the increased urinary excretion of C5b-9 was observed only in PHN rats. Moreover, the time of urinary C5b-9 complex excretion was earlier than that of urinary protein in the rats with PHN. CONCLUSION: Urinary C5b-9 complex excretion could be taken as one of several sensitive immunologic parameters in diagnosing of PHN and in distinguishing PHN from other type of nephritis.

关 键 词：肾炎 大鼠 尿液 补体 C5b-9复合物 比较研究 

分 类 号：R692.3[医药卫生—泌尿科学]