凝血酶、花生四烯酸及阿司匹林对中性粒细胞与血小板之间粘附的影响  

Effects of thrombin, arachidonic acid and aspirin on leukocyte-platelet adhesion

作　　者：吴蓝鸥[1] 沈志强[2] 吴平[3] 陈植和[2] 刘吉开[4] 

机构地区：[1]昆明医学院基础药学院药理学教研室,云南昆明650031 [2]云南省天然药物药理学重点实验室,昆明医学院云南昆明650031 [3]昆明医学院第二附属医院耳鼻咽喉科,云南昆明650101 [4]中科院昆明植物研究所开放实验室,云南昆明650204

出　　处：《中国病理生理杂志》2003年第1期107-109,共3页Chinese Journal of Pathophysiology

基　　金：云南省自然科学基金资助项目 (No 98C0 66M)

摘　　要：目的 :探讨不同浓度的凝血酶、花生四烯酸 (AA)和阿司匹林对大鼠血小板与中性粒细胞之间粘附作用的影响。方法 :应用改良的Hamburger等和沈志强等方法观察大鼠血小板与中性粒细胞之间的粘附作用。结果 :凝血酶在 50U/L就能促进血小板中性粒细胞之间的粘附 ,且随浓度增加而增大 ,在 30 0U/L时达最大作用 ,再随着浓度的增加 ,粘附率反而下降。 2 5μmol/L的AA即表现为促进细胞间粘附作用 ,并随浓度的增加而增强 ,于 1 0 0μmol/L时达最大作用 ,若再增加浓度 ,则粘附率明显下降 ;阿司匹林对上述两种血小板激活剂引起的血小板 -中性粒细胞间的粘附具有明显抑制作用 ,且呈浓度相关性 ,其IC50 分别为 62 9和 51 4μmol/L。 结论 :凝血酶和AA在一定范围内呈浓度依赖性促进血小板 -中性粒细胞的粘附作用 ,但浓度再增加 ,则粘附率反而下降 ;阿司匹林可明显抑制凝血酶和AA引起的血小板AIM: To investigate the effects of different concentrations of thrombin, arachidonic acid (AA) and aspirin on leukocyte-platelet adhesion. METHODS: The methods of Hamburger et al and Shen Zhi-qiang et al were used to study the adhesion between platelets and leukocytes in rats. RESULTS: 50 U/L of thrombin markedly stimulated the binding between platelets and leukocytes; the efficacy of thrombin increased with its concentration and produced the maximum effect at 300 U/L. But the adhesion rate decreased while the concentration of thrombin was more than 300 U/L. 25 μmol/L of AA significantly enhanced the binding of platelets to leukocytes; the efficacy of AA increased with its concentration and obtained the maximum effect at 100 μmol/L. The adhesion rate, however, decreased while the concentration of AA was more than 100 μmol/L. Aspirin could inhibit thrombin-or AA-induced adhesion between platelets and leukocytes. CONCLUSION: It is suggested that thrombin and AA, in a certain range of concentrations, concentration-dependently induced the binding of platelets to leukocytes; the adhesive rate, however, decreased as the concentration of the above inducers increased. Aspirin could inhibit platelet-leukocyte adhesion stimulated by thrombin and AA.

关 键 词：凝血酶 花生四烯酸 阿司匹林 中性粒细胞 血栓性疾病 血小板粘附 

分 类 号：R543[医药卫生—心血管疾病] R973.2[医药卫生—内科学]