心脏保存后冠脉流量减少机制的实验研究  

The mechanism of decrease in coronary flow after the isolated rat heart preservation

作　　者：刘相燕[1] 宋惠民[2] 毕研文[2] 董华进[3] 

机构地区：[1]山东省立医院胸外科,山东济南250021 [2]山东大学齐鲁医院心外科,山东济南250012 [3]北京军事医学科学院,北京100036

出　　处：《中国病理生理杂志》2003年第1期113-115,共3页Chinese Journal of Pathophysiology

摘　　要：目的 :探讨离体心脏低温较长时间保存后冠脉流量减少的发生机制。方法 :离体大鼠心脏在不同的保存液中 0℃ 8h保存后再灌注 ,应用作用于血管内皮及平滑肌的药物 ,观察冠脉流量 (CF)、冠脉血管阻力 (CVR)及再灌注前、后的水肿程度。结果 :心脏 0℃ 8h保存 -再灌注后 ,各实验组CF均降低 (P <0 0 1 ) ,CVR升高 (P <0 0 1 )。应用作用于血管内皮的药物 (5 -HT)后 ,山东医科大学心脏保存液 - 1号 (SDMC - 1 )组 ,Collin’s组 :CF降低 ;CVR升高。而Stanford组CF无变化 ,CVR升高 ;SDMC - 2组CF显著增加 (P <0 0 1 ) ,CVR降低 (P <0 0 5)。而应用作用于血管平滑肌的药物 (腺苷 )后 ,4组CF均增加 ,CVR均降低 (P <0 0 1 )。保存末 4组的水肿程度基本相同 ,再灌注后 ,Collin’s组水肿加重 ,Stanford组水肿显著加重 ,SDMC - 1、2组无变化。结论AIM: To clarify the possible mechanism of decrease in coronary flow (CF) after long-term hypothermic storage of the isolated rat hearts. METHODS: The isolated rat hearts preserved in different solutions (SDMC-1,2 S., Stanford S., Collin's S.) at 0℃ for 8 hours were reperfused with 5-hydroxytryptamine (5-HT)(10 -4 mmol/L) and adenosine(Ade)(5 mmol/L) respectively, and the coronary flow(CF), coronary vascular resistance(CVR) and the weight of heart were measured before and after reperfusion. RESULTS: CF decreased while CVR increased in all groups after storage-reperfusion. When endothelium-dependent vasodilator (5-HT) was used, CF in SDMC-2 group was highly increased and CVR decreased. Whereas CF was significantly decreased and CVR increased in Collin's group( P <0 01). Furthermore, after the administration of Ade, CF was increased in all groups. CONCLUSION: Coronary artery endothelium injury is involved in the mechanism of decrease in coronary flow.

关 键 词：心脏保存 冠脉流量减少 实验研究 

分 类 号：R331.31[医药卫生—人体生理学]