1-磷酸甘露醇脱氢酶基因转化水稻的研究  

Transformation of Rice with mtlD Gene

作　　者：王慧中[1] 刘俊君[2] 卢德赵[3] 赵艳 颜美仙[3] 钱前[3] 彭学贤[2] 陈受宜[2] 黄大年[3] 

机构地区：[1]杭州师范学院生物化学与分子生物学重点实验室,浙江杭州310036 [2]中国科学院遗传与发育生物学研究所植物生物技术开放实验室,北京100101 [3]中国水稻研究所,浙江杭州310006 [4]杭州商学院,浙江杭州310035

出　　处：《中国水稻科学》2003年第1期6-10,共5页Chinese Journal of Rice Science

基　　金：浙江省自然科学基金资助项目(3982 97;30 2 0 61 );国家自然科学基金资助项目(39970 4 0 9);中国水稻科学发展基金资助项目 (0 0 0 32 39)

摘　　要：PCR和 Southern blotting检测表明 ,来自大肠杆菌的 mtl D基因已通过农杆菌介导整合进水稻基因组。mtl D基因在 T1代出现分离 ,T2 代出现纯系。在 0 .75% Na Cl胁迫下 ,7个转基因 T3 代株系都能检测到 mtl D酶活性 ,与对照相比细胞膜的相对电导率和大分子渗漏值明显降低。部分转基因株系能在 1 .0 % Na Cl浓度下正常生长 ,而对照在 0 .5% Na Cl浓度下已不能存活。通过有性杂交途径实现了 mtl D和 gut D两个基因的聚合 ,部分杂交后代株系能在 1 .2 5% Na Cl胁迫下正常生长结实。PCR and Southern blot analysis indicated that mtlD gene had been integrated into the rice genome mediated by Agrobacterium tumefaciens LBA4404 (pBIM). mtlD gene segragated in T 1 generation and pure transgenic lines appeared in T 2 generation. The expression of the mtlD gene in transgenic plants was demonstrated by enzymatic activity assay. Under 0 75% NaCl stress, the relative electronic conductivity and membrane permeability were detected in T 3 transgenic plants of 7 different lines. Results showed that the damage to membrane structure of the transgenic plant was lower than that of their controls. Some transgenic plant lines could grow normally under 1.0% NaCl stress whereas the controls could not grow and died after 2 weeks under the same environment. Through sexual hybridization, rice plants with mtlD gene and gutD gene were got, some of them could grow normally under the stress of 1.25% NaCl.

关 键 词：水稻 1-磷酸甘露醇脱氢酶基因 6-磷酸山梨醇脱氢酶基因 转基因植株 耐盐性 产量性性 有性杂交 农艺性状 

分 类 号：S511[农业科学—作物学] S336