利用标记基因检测转Bt基因棉种子纯度的研究  

Technique for Testing Seeds Purity of Bt Transgenic Cotton by Using Marker Gene

作　　者：李长福[1] 吴振廷[1] 王学林[1] 孟海[1] 林华峰[1] 

机构地区：[1]安徽农业大学植保系,合肥230036

出　　处：《安徽农业大学学报》2003年第1期23-26,共4页Journal of Anhui Agricultural University

基　　金：安徽省自然科学基金项目(01041402)资助。

摘　　要：根据转Bt基因棉"国抗1号"转入的外源基因中整合有GUS基因和NPT 基因,建立了3种种子纯度检测方法:GUS组织化学染色法、NPT 培养基法和NPT 叶片涂抹法,准确率都达到98%以上;在检测转Bt基因棉原始群系"20-1"时与生物测定比较准确率也在98%以上,因此这3种方法可用于转Bt基因棉大规模制种过程中和推广过程中良种纯度的检测,相应方法同样适用于基因组中整合有GUS基因和(或)NPT 基因的其它转Bt基因棉的品种。According to GUS gene and NPTⅡgene transformed into Bt transgenic cotton cultivar 'Guokang mian No1',three methods for testing the purity of the cotton seeds were established.The results indicated that the accurate rates were up to 98%,and the accurate rates were also up to 98% (compared to the result of bioassay) in testing the original Bt transgenic cotton group '201'.The corresponding methods were suitable to every kind of Bt transgenic cotton cultivar with GUS gene or (and) NPTⅡgene.

关 键 词：转BT基因棉 标记基因 GUS基因 NPTⅡ基因 种子纯度 检测方法 

分 类 号：S562[农业科学—作物学]