用核酸探针技术检测脊尾白虾体内的白斑综合症病毒  

Detecting White Spot Syndrome Virus (WSSV) in Exopalaemon carinicauda by Dot Blot Hybridization and In Situ Hybridization

作　　者：雷质文[1] 黄劼 梁艳[2] 刘莉[2] 俞开康[3] 

机构地区：[1]青岛出入境检验检疫局,青岛266002 [2]中国水产研究院黄海水产研究所 [3]青岛海洋大学水产学院

出　　处：《安徽农业大学学报》2003年第1期82-85,共4页Journal of Anhui Agricultural University

基　　金：国家重点基础研究规划项目(G1999012002);"九五"攻关项目(96-005-03-01-02)资助。

摘　　要：用斑点杂交和原位杂交检测了从病虾池捕获的脊尾白虾体内的WSSV。8尾脊尾白虾的斑点杂交检测阳性率为100%;脊尾白虾胃的上皮组织和结缔组织、位于鳃区头胸甲处的角化上皮、头胸甲后缘与虾体相连的角化上皮、结缔组织、附肢处的角化上皮、鳃丝的柱状上皮及其腔隙内的血淋巴、肝胰腺小管间的上皮组织及其血窦内的血淋巴、头胸甲的肌肉、造血组织、脊尾白虾精荚之结缔组织细胞、卵巢的结缔组织及其滤泡细胞原位杂交呈阳性。以上结果进一步证实了脊尾白虾是WSSV的天然宿主。In the present study, white spot syndrome virus (WSSV) in Exopalaemon carinicauda which were captured in ponds,and WSSV was detected by Dot Blot hybridization and In Situ hybridization. WSSV was detected in all 8 Exopalaemon carinicauda which were captured in ponds by dot blot hybridization.WSSVpositive wells were observed in cuticular epidermis, stomach epithelium, gill, hematopoietic tissue, loose connective tissue and hepatopancreas epithelium of Exopalaemon carinicauda.Follicle cells and connective cells in the ovaries and connective cells in the testis of Exopalaemon carinicauda were WSSV positive by in situ hybridization.

关 键 词：核酸探针技术 检测 脊尾白虾 白斑综合症病毒 WSSV 斑点杂交 原位杂交 

分 类 号：S945.49[农业科学—水产养殖]